A rapid method for demonstrating skeletal muscle motor innervation in frozen sections.
A rapid method for demonstrating skeletal muscle motor innervation in frozen sections.
复制标题
一种在冰冻切片中演示骨骼肌运动神经支配的快速方法。
作者：
J. Toop
在涂有3% EDTA的载玻片上取50-100 mm厚的纵向冷冻肌肉切片，干燥后孵育以显示乙酰胆碱酯酶。随后在0.5% K3Fe(CN)6中孵育，然后在福尔钙或福尔盐水中固定30分钟。洗涤后，载玻片在含0.1% CuSO4的20% AgNO3水溶液中37℃孵育2-30分钟。在1%的喹啉（w/v）溶液中孵育，相对于NaSO3 (w/v) 5%的喹啉（w/v）溶液中孵育，轴突和亚神经装置染成深棕色至黑色，而肌肉纤维和细胞核染色较差。该方法允许在接受样本后3 1/ 4 -4小时内研究骨骼肌神经肌肉支配的模式，并可以确定末梢神经支配比例。
Longitudinal 50-100 mum-thick frozen sections of muscle are picked up on slides coated with 3% EDTA and after drying are incubated to demonstrate acetylcholinesterase. Subsequent incubation in 0.5% K3Fe(CN)6 is followed by fixation for 30 minutes in formol-calcium or formol-saline. After washing, the slides are incubated in 20% aqueous AgNO3 containing 0.1% CuSO4 for 2-30 minutes at 37 C. Following development in a 1% solution of quinol (w/v) 5% with respect to NaSO3 (w/v), axons and subneural apparatus stain dark brown to black in contrast to the less well stained muscle fibers and nuclei. This procedure permits study of the pattern of neuromuscular innervation in skeletal muscle 3 1/2-4 hours after receipt of a sample, and makes possible determination of the terminal innervation ratio.