Porcine wound models for skin substitution and burn treatment
Porcine wound models for skin substitution and burn treatment
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DOI：
10.1016/s0142-9612(03)00502-7
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发表时间：
2004-04-01
期刊：
影响因子：
14
通讯作者：
Ulrich, MMW
中科院分区：
文献类型：
--
作者：
Middelkoop, E;van den Bogaerdt, AJ;Ulrich, MMW
皮肤再生是组织工程的一个重要领域。特别是在较大的烧伤和慢性伤口中，目前的治疗方法不足以防止疤痕形成和促进愈合。通常通过体外测试来初步筛选可能感兴趣的皮肤替代产品。然而，在进入临床之前，有必要对免疫活性动物进行体内研究，以证明疗效并提供安全方面的信息。我们以家猪为试验动物，在皮肤替代材料的研究中获得了丰富的经验，包括组织工程皮肤替代品和烧伤创面治疗。描述了两种模型：用于测试真皮和表皮替代品的切除伤口模型和用于接触性和烫伤烧伤的烧伤模型。它可以将现代伤口敷料与目前烧伤治疗的黄金标准进行比较。这些实验的结果表明，体内测试能够揭示体外研究中未检测到的治疗的优点。 (C) 2003 Elsevier Ltd. 保留所有权利。
Skin regeneration is an important field of tissue engineering. Especially in larger burns and chronic wounds, present treatments are insufficient in preventing scar formation and promoting healing. Initial screening of potentially interesting products for skin substitution is Usually done by in vitro tests. Before entering the clinic, however, in vivo studies in immunocompetent animals are necessary to prove efficacy and provide information on safety aspects.We have obtained extensive experience using the domestic pig as test animal for studies on skin replacement materials, including tissue engineered skin substitutes, and burn wound treatment.Two models are described: an excisional wound model for testing of dermal and epidermal substitutes and a burn wound model for contact and scald burns. which allows testing of modern wound dressings in comparison to the present gold standards in burn treatment. The results of these experiments show that in vivo testing was able to reveal (dis)advantages of the treatments which were not detected during in vitro Studies. (C) 2003 Elsevier Ltd. All rights reserved.