Gene Composer in a structural genomics environment.
Gene Composer in a structural genomics environment.
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结构基因组学环境中的 Gene Composer。
DOI：
10.1107/s1744309111027424
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发表时间：
2011
期刊：
影响因子：
--
通讯作者：
Stewart,Lance
中科院分区：
文献类型：
--
作者：
Lorimer,Don;Raymond,Amy;Mixon,Mark;Burgin,Alex;Staker,Bart;Stewart,Lance
西雅图传染病结构基因组学中心(SSGCID)的结构基因组学工作需要操作大量的氨基酸序列和潜在的DNA序列，这些序列将被克隆到表达载体中。为了提高高通量蛋白质结构测定的效率，人们开发了一个数据库软件包，Gene Composer，它可以方便地进行信息丰富的蛋白质结构及其基因序列的设计。凭借其模块化的工作流程设计和众多的图形用户界面，Gene Composer使研究人员能够执行现代结构引导蛋白质工程和合成基因工程中使用的所有常见生物信息学步骤。给出了H1N1依赖于RNA的RNA聚合酶PB2亚基结构测定的实例。
The structural genomics effort at the Seattle Structural Genomics Center for Infectious Disease (SSGCID) requires the manipulation of large numbers of amino-acid sequences and the underlying DNA sequences which are to be cloned into expression vectors. To improve efficiency in high-throughput protein structure determination, a database software package, Gene Composer, has been developed which facilitates the information-rich design of protein constructs and their underlying gene sequences. With its modular workflow design and numerous graphical user interfaces, Gene Composer enables researchers to perform all common bioinformatics steps used in modern structure-guided protein engineering and synthetic gene engineering. An example of the structure determination of H1N1 RNA-dependent RNA polymerase PB2 subunit is given.