Subunit composition of ribosome in the yqgF mutant deficient in pre-16S rRNA processing of Escherichia coli.
Subunit composition of ribosome in the yqgF mutant deficient in pre-16S rRNA processing of Escherichia coli.
复制标题
大肠杆菌前 16S rRNA 加工缺陷的 yqgF 突变体中核糖体的亚基组成。
DOI：
10.1159/000494494
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发表时间：
2018
影响因子：
1.2
通讯作者：
J.
中科院分区：
文献类型：
--
作者：
Kurata;T.;Nakanishi;S.;Hashimoto;M.;Taoka;M.;Isobe;T.;and Kato;J.
大肠杆菌16 S、23 S和5S核糖体RNA（rRNA）被转录为单个初级转录物，其随后被几种RNA酶加工成成熟的rRNA。据报道，三种RNase（RNase III、RNase E和RNase G）在前体16 S rRNA（pre-16 S rRNA）的5′-前导序列的加工中起作用。之前，我们发现一种新的必需YqgF参与了该过程。我们利用LC-MS/MS分析了yqgF ts突变体的核糖体亚基，发现突变体核糖体中核糖体蛋白S1的拷贝数减少，表明yqgF基因通过加工前16 SrRNA的5′端，使核糖体蛋白S1进入核糖体。核糖体蛋白S1在大肠杆菌中是翻译所必需的。大肠杆菌;因此，我们的研究结果表明，YqgF在核糖体组装的最后一步将新合成的核糖体的无活性形式转化为活性形式。
Escherichia coli 16S, 23S, and 5S ribosomal RNAs (rRNAs) are transcribed as a single primary transcript, which is subsequently processed into mature rRNAs by several RNases. Three RNases (RNase III, RNase E, and RNase G) were reported to function in processing the 5′-leader of precursor 16S rRNA (pre-16S rRNA). Previously, we showed that a novel essential YqgF is involved in that processing. Here we investigated the ribosome subunits of the yqgF ts mutant by LC-MS/MS. The mutant ribosome had decreased copy numbers of ribosome protein S1, suggesting that the yqgF gene enables incorporation of ribosomal protein S1 into ribosome by processing of the 5′-end of pre-16S rRNA. The ribosome protein S1 is essential for translation in E. coli; therefore, our results suggest that YqgF converts the inactive form of newly synthesized ribosome into the active form at the final step of ribosome assembly.