Laboratory-Scale Production of Sterile Targeted Microbubbles.
Laboratory-Scale Production of Sterile Targeted Microbubbles.
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DOI：
10.1007/978-1-0716-1811-0_31
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发表时间：
2022
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全氟化碳充气微泡在临床上用作超声造影剂。我们一直在开发基于 BACS（浮力激活细胞分选）或 BUBLES（浮力激活分离）的靶向微泡，用于从血液中离体细胞分离，用于循环肿瘤细胞 (CTC) 检测和造血细胞分离。最近，我们进一步将靶向微泡应用于多标记细胞分选，并利用脂壳微泡的许多有趣特性，将其作为人工抗原呈递细胞（aAPC）用于T细胞激活和扩增。本章将描述用于研究应用的无菌目标微泡的制造过程。
Perfluorocarbon gas-filled microbubbles are clinically used as ultrasound contrast agents. We have been developing targeted microbubbles based BACS (buoyancy activated cell sorting) or BUBLES (buoyancy enabled separation) for ex vivo cell isolation from bloods for circulating tumor cell (CTC) detection and hematopoietic cell isolation. Recently, we further applied targeted microbubbles for multi-marker cell sorting, and as artificial antigen presenting cells (aAPC) for T cell activation and expansion by taking advantage of a number of interesting properties of lipid-shelled microbubbles. This chapter will describe the process of manufacturing sterile targeted microbubbles for research applications.