Cytometric analysis reveals an association between allergen-responsive natural killer cells and human peanut allergy.
Cytometric analysis reveals an association between allergen-responsive natural killer cells and human peanut allergy.
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细胞仪分析揭示了过敏原反应性天然杀伤细胞与人类花生过敏之间的关联。
DOI：
10.1172/jci157962
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发表时间：
2022-10-17
影响因子：
15.9
通讯作者：
Nadeau, Kari C.
中科院分区：
文献类型：
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作者：
Zhou, Xiaoying;Yu, Wong;Dunham, Diane M.;Schuetz, Jackson P.;Blish, Catherine A.;DeKruyff, Rosemarie H.;Nadeau, Kari C.
食物过敏是过敏性反应的主要原因，先天免疫系统和适应性免疫系统中的过敏原特异性免疫反应在其发病机制中起关键作用。我们对非过敏（NA）和花生过敏（PA）受试者的免疫细胞反应进行了全面的表型和功能研究，发现NK细胞活化与PA受试者对过敏原的免疫反应密切相关。花生反应性NK细胞在PA参与者中表现出与NA参与者不同的表达模式。过敏原激活的NK细胞同时表达Th2和免疫调节细胞因子，提示其在介导和调节Th2过敏反应中具有潜在的功能作用。CD3+ T细胞的缺失减弱了NK细胞对花生过敏原刺激的反应，表明花生反应性NK细胞依赖于T细胞。我们还发现口服免疫治疗与体外花生过敏原刺激的NK反应降低有关。这些结果表明NK细胞与食物过敏免疫反应有关，这种动员细胞群的规模表明它们在过敏免疫中发挥功能作用。
Food allergies are a leading cause of anaphylaxis, and allergen-specific immune responses in both the innate and the adaptive immune system play key roles in its pathogenesis. We conducted a comprehensive phenotypic and functional investigation of immune cell responses from nonallergic (NA) and peanut allergic (PA) participants cultured with media alone or peanut protein and found, surprisingly, that NK cell activation was strongly associated with the immune response to allergen in PA participants. Peanut-responsive NK cells manifested a distinct expression pattern in PA participants compared with NA participants. Allergen-activated NK cells expressed both Th2 and immune regulatory cytokines, hinting at a potential functional role in mediating and regulating the Th2 allergic response. Depletion of CD3+ T cells attenuated the response of NK cells to peanut-allergen stimulation, suggesting that peanut-responsive NK cells are T cell dependent. We also showed that oral immune therapy was associated with decreased NK responses to peanut allergen stimulation in vitro. These results demonstrate that NK cells are associated with the food-allergic immune response, and the magnitude of this mobilized cell population suggests that they play a functional role in allergic immunity.