Administration of the Rho-Kinase Inhibitor Fasudil Before Ischemia or just After Reperfusion, but not 30 min After Reperfusion, Protects the Stunned Myocardium in Swine
Administration of the Rho-Kinase Inhibitor Fasudil Before Ischemia or just After Reperfusion, but not 30 min After Reperfusion, Protects the Stunned Myocardium in Swine
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DOI：
10.1007/s10557-008-6106-y
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发表时间：
2008-05
影响因子：
3.4
通讯作者：
I. Shibata;O. Yoshitomi;Tadasuke Use;H. Ureshino;Sungsam Cho;T. Maekawa;T. Hara;K. Sumikawa
中科院分区：
文献类型：
--
作者：
I. Shibata;O. Yoshitomi;Tadasuke Use;H. Ureshino;Sungsam Cho;T. Maekawa;T. Hara;K. Sumikawa
ObjectivesWe assessed the effects of administration time for fasudil treatment of stomach myocardium in 40 anesthesized open chest sinus.Materials and methodsAll猪受到12分钟的缺血，然后再灌注产生顿抑心肌。A组（n= 11）在缺血前和再灌注后均给予生理盐水代替法舒地尔。B组（n= 10）于缺血前45 min开始以13 μg/kg/min的速度静脉注射法舒地尔30 min，再灌注后给予生理盐水。C组（n= 10）和D组（n= 9）在缺血前给予生理盐水，C组在再灌注前即刻和D组在再灌注后30 min分别给予法舒地尔13 μg kg− 1 min − 1。两组治疗时间均为30 min。结果和讨论A组3只猪，B和C组各2只猪，D组1只猪在再灌注后出现室颤或心动过速，并从进一步分析中排除。B组和C组再灌注90 min时%SS较基线的变化分别为68 ± 8%和75 ± 8%，显著高于A组和D组（47 ± 10%和43 ± 8%）。
ObjectivesWe assessed the effect of administration time for fasudil treatment of the stunned myocardium in 40 anesthetized open chest swine.Materials and methodsAll swine were subjected to 12 min ischemia followed by reperfusion to generate stunned myocardium. Group A (n= 11) received saline in place of fasudil both before ischemia and after reperfusion. Group B (n= 10) received 30 min intravenous fasudil at a rate of 13 μg/kg/min starting 45 min before ischemia and received saline after reperfusion. Groups C (n= 10) and D (n= 9) received saline before ischemia, and received fasudil at a rate of 13 μg kg−1min−1starting just before reperfusion in group C and 30 min after reperfusion in group D. In both groups, treatment lasted 30 min. Myocardial contractility was assessed by percent segment shortening (%SS).Results and discussionThree swine in group A, 2 swine in each of groups B and C, and one swine in group D had ventricular fibrillation or tachycardia after reperfusion and were excluded from further analysis. The changes of %SS from baseline at 90 min after reperfusion in groups B and C were 68 ± 8% and 75 ± 8%, respectively, which were significantly higher than in group A or D (47 ± 10% or 43 ± 8%).ConclusionWe conclude that fasudil administered before ischemia or just after reperfusion, but not 30 min after reperfusion, protects the stunned myocardium.