Ultrahigh-pressure liquid chromatography using a 1-mm id column packed with 1.5-μm porous particles
Ultrahigh-pressure liquid chromatography using a 1-mm id column packed with 1.5-μm porous particles
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DOI：
10.1002/jssc.200600535
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发表时间：
2007-05-01
影响因子：
3.1
通讯作者：
Colon, Luis A.
中科院分区：
文献类型：
--
作者：
Anspach, Jason A.;Maloney, Todd D.;Colon, Luis A.
色谱的发展导致用于制造HPLC柱的填充材料的尺寸减小。当柱背压超过10 000 psi（类似于700 bar）时，所需背压的增加导致该技术被称为超高压液相色谱（UHPLC）。直到最近，填充有sub-2- tm材料的色谱柱通常分为两类：设计用于压力小于5000 psi（350 bar）的传统HPLC系统的短（小于5 cm）色谱柱，或毛细管色谱柱（内径小于100 pin）。通过使用直径< 2 μ m的填充材料来制造UHPLC柱，效率增加并且分析时间减少，这与填充材料的尺寸成正比。然而，为了实现和利用效率的增加，柱必须保持通常与分析柱相关的长度（15-25 cm）。我们用1.5 μ m的填料填充了直径为1 mm、长度为150 mm的色谱柱，并评价了它们在UHPLC中的性能。在板高度与线速度的曲线图中，达到最佳线速度所需的压力在1104巴（16000 psi）附近。1.5 μ m的颗粒填充柱进行了比较，与更传统的150分钟长的分析柱填充3 Am材料。该柱显示出的效率约为3 μ m填充柱的两倍，并且理论上预测分析时间随之减少。
The evolution of chromatography has led to the reduction in the size of the packing materials used to fabricate HPLC columns. The increase in the backpressure required has led to this technique being referred to as ultrahigh-pressure liquid chromatography (UHPLC) when the column backpressure exceeds 10 000 psi (similar to 700 bar). Until recently, columns packed with sub-2- tm materials have generally fitted into two classes; either short (less than 5 cm) columns designed for use on traditional HPLC systems at pressures less than 5000 psi (350 bar), or capillary columns (inner diameters less than 100 pin). By using packing materials with diameters < 2 mu m to fabricate UHPLC columns, there is an increase in efficiency and a decrease in the analysis time that are directly proportional to the size of the packing material. In order to realize and exploit the increase in efficiency, however, the columns must maintain lengths typically associated with analytical columns (15-25 cm). We have packed 1 mm diameter, 150 mm in length columns with 1.5 mu m packing material, and evaluated their performance in UHPLC. The pressure required to achieve optimum linear velocities in plots of plate height versus linear velocity was in the vicinity of 1104 bar (16 000 psi). The 1.5 mu m particle-packed column was compared with the more traditional 150 min long analytical columns packed with 3 Am materials. This column showed an efficiency that was approximately twice that observed with the 3 mu m packed column and a concomitant reduction in the analysis time, theoretically predicted.