Assembly of Fe3O4 nanoparticles on PEG-functionalized graphene oxide for efficientmagnetic imaging and drug delivery
Assembly of Fe3O4 nanoparticles on PEG-functionalized graphene oxide for efficientmagnetic imaging and drug delivery
复制标题
Fe3O4 纳米粒子在 PEG 功能化氧化石墨烯上的组装，用于有效的磁成像和药物输送
DOI：
10.1039/c5ra09901c
复制
发表时间：
2015
期刊：
影响因子：
3.9
通讯作者：
Xiuwen Zheng
中科院分区：
文献类型：
--
作者：
Weihong Chen;Xin Wen;Guorong Zhen;Xiuwen Zheng
报道了在PEG-功能化的氧化石墨烯上组装Fe 3 O 4纳米颗粒（NPs）用于有效的磁成像和药物递送。首先合成纳米级氧化石墨烯（GO），并通过支化的生物相容性聚乙二醇（PEG）官能化，以在生理溶液中提供高的水溶性和稳定性。接下来，在1-乙基-3-（3-二甲基氨基丙基）碳二亚胺（EDC）的存在下，通过形成酰胺键将内消旋-2，3-二巯基琥珀酸改性的Fe 3 O 4 NP锚定到GO片上。结果表明，Fe 3 O 4-PEG-GO纳米复合材料具有良好的生理稳定性和较低的细胞毒性。此外，研究了芳香族药物阿霉素（DOX）通过π-π堆积和疏水作用在Fe 3 O 4-PEG-GO纳米复合材料上的可控负载。结果表明，负载有抗癌药物的Fe 3 O 4-PEG-GO/DOX显示出显著高的细胞毒性。Fe 3 O 4-PEG-GO/DOX纳米复合材料显示出显著增强的细胞MRI，能够在铁浓度为10 μg mL−1时检测细胞，细胞密度为2 × 105个细胞/mL。目前的工作提供了一种通用的方法，合理设计和合成的多功能治疗诊断纳米平台的基础上Fe 3 O 4-PEG-GO纳米颗粒具有良好的生物相容性和能力，同时进行成像和治疗的临床结果。
Assembly of Fe3O4 nanoparticles (NPs) on PEG-functionalized graphene oxide for efficient magnetic imaging and drug delivery are reported. Nanoscale graphene oxide (GO) was first synthesized and functionalized by branched, biocompatible polyethylene glycol (PEG) to render high aqueous solubility and stability in physiological solutions. Next, the meso-2,3-dimercaptosuccinnic acid-modified Fe3O4 NPs were anchored onto GO sheets via formation of an amide bond in the presence of 1-ethyl-3-(3-dimethyaminopropyl)carbodiimide (EDC). It was found that the Fe3O4–PEG–GO nanocomposites possess good physiological stability and low cytotoxicity. Furthermore, controlled loading of aromatic drugs, doxorubicin (DOX), a typical anticancer drug, onto the Fe3O4–PEG–GO nanocomposites via π–π stacking and hydrophobic interactions is investigated. It is demonstrated that Fe3O4–PEG–GO/DOX loaded with the anticancer drug shows remarkably high cytotoxicity. The Fe3O4–PEG–GO/DOX nanocomposites show significantly enhanced cellular MRI, being capable of detecting cells at the iron concentration of 10 μg mL−1 with cell density of 2 × 105 cells per mL. The current work provides a general approach toward rational design and synthesis of a versatile theranostic nanoplatform based on Fe3O4–PEG–GO NPs with good biocompatibility and the capability of simultaneously performing imaging and therapy for clinical outcomes.