The F1FOATP synthase genes in Methanosarcina acetivorans are dispensable for growth and ATP synthesis
The F1FOATP synthase genes in Methanosarcina acetivorans are dispensable for growth and ATP synthesis
复制标题
DOI：
10.1111/j.1574-6968.2009.01785.x
复制
发表时间：
2009-11-01
影响因子：
2.1
通讯作者：
Mueller, Volker
中科院分区：
文献类型：
--
作者：
Saum, Regina;Schlegel, Katharina;Mueller, Volker
基于生物化学和基因组数据，在文献中有一个长期的讨论，一些古细菌物种是否可能在一个细胞中具有两种结构和功能不同的ATP酶：古细菌Lambda（1）Lambda（O）和细菌F1FO ATP合酶。为了解决细菌F1FO ATP合酶的潜在作用，我们已经将产乙酸甲烷八叠球菌中的F1FO ATP酶基因簇与嘌呤霉素抗性盒交换。有趣的是，突变体能够在生长动力学与野生型没有差异的情况下生长，并且野生型和突变体中的细胞ATP含量相同。这些数据表明，F1FO ATP合酶对M.食醋动物。
There is a long-standing discussion in the literature, based on biochemical and genomic data, whether some archaeal species may have two structurally and functionally distinct ATP synthases in one cell: the archaeal Lambda(1)Lambda(O) together with the bacterial F1FO ATP synthase. To address a potential role of the bacterial F1FO ATP synthase, we have exchanged the F1FO ATPase gene cluster in Methanosarcina acetivorans against a puromycin resistance cassette. Interestingly, the mutant was able to grow with no difference in growth kinetics to the wild type, and cellular ATP contents were identical in the wild type and the mutant. These data demonstrate that the F1FO ATP synthase is dispensable for the growth of M. acetivorans.