Silver ion-enhanced particle-specific cytotoxicity of silver nanoparticles and effect on the production of extracellular secretions of Phanerochaete chrysosporium
Silver ion-enhanced particle-specific cytotoxicity of silver nanoparticles and effect on the production of extracellular secretions of Phanerochaete chrysosporium
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银离子增强纳米银颗粒特异性细胞毒性及其对黄孢原毛平革菌细胞外分泌物产生的影响
DOI：
10.1016/j.chemosphere.2017.12.185
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发表时间：
2018-04-01
期刊：
影响因子：
8.8
通讯作者：
Hu, Liang
中科院分区：
文献类型：
--
作者：
Huang, Zhenzhen;Xu, Piao;Hu, Liang
研究了银离子（Ag+）对银纳米颗粒（AgNPs）对黄孢原毛革菌（Acantherochaete chrysosporium）细胞毒性的影响，并观察了毒素胁迫下胞外分泌物的变化。草酸的生产引起与中等浓度的2，4-二氯苯酚（2，4-DCP）和银纳米粒子达到一个平台，分别在10毫克/升和10 μ M。草酸积累的增加伴随着较高的锰过氧化物酶（MnP）和木质素过氧化物酶（LiP）的活性。然而，草酸，MNP和LiP的分泌显着抑制由于Ag+掺入AgNP溶液。随着2，4-DCP浓度的增加，胞外多聚物（EPS）的产生量显著增加，但在100 mg/L 2，4-DCP处理2,4 h后，EPS的产生量明显减少，说明部分EPS可作为碳源和能源消耗，改善生物对毒性胁迫的耐受性。此外，银纳米粒子诱导的“颗粒特异性”的细胞毒性大大增强与额外的银+证明了其显着的负面影响，细胞生长，质膜的完整性，和形态保存相比，在相同的银浓度的银纳米粒子。(C)2017爱思唯尔有限公司版权所有
This study investigated the influence of silver ions (Ag+) on the cytotoxicity of silver nanoparticles (AgNPs) in Phanerochaete chrysosporium and noted the degree of extracellular secretions in response to the toxicant's stress. Oxalate production was elicited with moderate concentrations of 2,4-dichlorophenol (2,4-DCP) and AgNPs reaching a plateau at 10 mg/L and 10 mu M, respectively. Increased oxalate accumulation was accompanied by higher activities of manganese peroxidase (MnP) and lignin peroxidase (LiP). However, the secretion of oxalate, MnP and LiP was significantly inhibited owing to Ag+ incorporation into AgNP solution. Production of extracellular polymeric substances (EPS) significantly elevated with an increase in 2,4-DCP concentrations; however, after 24 h of exposure to 100 mg/L 2,4-DCP, an obvious decrease in EPS occurred, indicating that part of EPS could be consumed as carbon and energy sources to ameliorate biological tolerance to toxic stress. Furthermore, AgNP-induced "particle-specific" cytotoxicity was substantially enhanced with additional Ag+ as evidenced by its significant negative impact on cellular growth, plasma membrane integrity, and morphological preservation compared with AgNPs at equal Ag concentration. (C) 2017 Elsevier Ltd. All rights reserved.