Effect of linkage of transduction domain sequences to a lymphoma idiotype DNA vaccine on vaccine effectiveness.
Effect of linkage of transduction domain sequences to a lymphoma idiotype DNA vaccine on vaccine effectiveness.
复制标题
转导结构域序列与淋巴瘤独特型 DNA 疫苗的连接对疫苗有效性的影响。
DOI：
10.1089/hyb.2006.25.306
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发表时间：
2006
期刊：
影响因子：
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通讯作者：
Solheim,JoyceC
中科院分区：
文献类型：
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作者：
Ashour,Abdel-Kader;Petersen,JasonL;McIlhaney,MaryM;Vose,JulieM;Solheim,JoyceC
用于治疗非霍奇金淋巴瘤的患者独特型特异性疫苗在临床试验中显示出希望，鼓励进一步提高独特型疫苗的有效性。先前已经发现，对于一些其他类型的实验疫苗，转导结构域的添加提高了疫苗免疫原性。转导结构域是能够增加通过细胞膜的转运的短氨基酸序列。在这项研究中，我们测试了小鼠B细胞38 C13淋巴瘤独特型DNA疫苗与人类免疫缺陷病毒（HIV）Tat衍生的转导序列对38 C13攻击的效力。独特型和转导结构域偶联独特型疫苗组的肿瘤发病率相似。在攻击后第22-23天，与仅接受独特型疫苗的组相比，接受由38 C13独特型序列加上修饰的达特转导序列组成的DNA疫苗的组中存活小鼠的数量显著更高。尽管在第24天后总体存活率差异无统计学意义，但接受独特型加Tat衍生转导结构域的小鼠存活率增加的趋势维持至攻击后第106天。因此，在独特型疫苗中添加促进细胞内转运的特定序列可能有可能提高此类疫苗的有效性。
Patient idiotype-specific vaccines for treatment of non-Hodgkin's lymphoma have shown promise in clinical trials, encouraging efforts to enhance the effectiveness of idiotype vaccines further. It has previously been found that for some other types of experimental vaccines, the addition of transduction domains has improved vaccine immunogenicity. Transduction domains are short amino acid sequences that are capable of increasing transport through cellular membranes. In this study, we tested murine B cell 38C13 lymphoma idiotype DNA vaccines with human immunodeficiency virus (HIV) Tat-derived transduction sequences for efficacy against 38C13 challenge. The rate of tumor onset was similar for the idiotype and transduction domain-conjugated idiotype vaccine groups. At days 22–23 postchallenge, the number of surviving mice was significantly higher in the group that had received a DNA vaccine consisting of the 38C13 idiotype sequence plus modified Tat transduction sequence, in comparison with the group that received idiotype-only vaccines. Although the overall survival difference was not statistically significant following day 24, a trend toward an increased survival rate for mice receiving idiotype plus Tat-derived transduction domains was maintained through day 106 postchallenge. Thus, the addition to idiotype vaccines of specific sequences that facilitate intracellular transport may have potential to improve the effectiveness of such vaccines.