Ontogeny of Na-K-ATPase activity in thick ascending limb and of concentrating capacity.
Ontogeny of Na-K-ATPase activity in thick ascending limb and of concentrating capacity.
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粗升肢Na-K-ATP酶活性和集中能力的个体发育。
DOI：
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发表时间：
1985
影响因子：
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通讯作者：
A. Aperia
中科院分区：
文献类型：
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作者：
S. Rane;A. Aperia
测定12日龄、16日龄、20日龄、30日龄和40日龄大鼠粗大升支(TAL)的Na-K-ATPase活性和尿浓缩能力。酶活性最显著的增加发生在16日龄到20日龄之间。在16日龄、20日龄和40日龄大鼠，皮质和延髓TAL中的酶活性之间的关系是相同的，大多数酶的测定都是在延髓TAL中进行的。TAL中Na-K-ATPase活性与尿浓缩能力的变化规律一致。Na-K-ATPase活性和浓缩能力在16日龄至20日龄期间的发育性增加伴随着血清皮质酮水平的升高，并被肾上腺切除所抵消。糖皮质激素处理可使13~16日龄大鼠的Na-K-ATPase活性和浓缩能力增加，但对17~20日龄大鼠的Na-K-ATPase活性无影响。从20日龄到40日龄，酶活性的增加伴随着单个肾单位肾小球滤过率的增加。结果表明，TAL中的Na-K-ATPase活性是发育过程中浓缩能力的重要决定因素。在16日龄至20日龄之间，Na-K-ATPase活性和浓缩能力的发育激增可能是由于血清皮质酮水平的上升所引起的。
Na-K-ATPase activity in the thick ascending limb of Henle (TAL) and the capacity to concentrate urine was determined in 12-, 16-, 20-, 30-, and 40-day-old rats. The most pronounced increase in enzyme activity occurred between 16 and 20 days of age. The relation between enzyme activity in the cortical and medullary TAL was found to be the same in 16-, 20-, and 40-day-old rats, and most enzyme determinations were made in the medullary TAL. The development of Na-K-ATPase activity in TAL and urinary concentrating capacity followed the same pattern. The developmental increase in Na-K-ATPase activity and concentrating capacity between 16 and 20 days of age was accompanied by an increase in serum corticosterone level and was abolished by adrenalectomy. Treatment with glucocorticoid hormones precociously induced Na-K-ATPase activity and concentrating capacity in 13- to 16-day-old rats but had no effect on Na-K-ATPase activity in 17- to 20-day-old rats. The increase in enzyme activity from 20 to 40 days of age was accompanied by an increase in the single nephron glomerular filtration rate. The results suggest that Na-K-ATPase activity in the TAL is an important determinant of the concentrating capacity during development. The developmental surge in Na-K-ATPase activity and concentrating capacity between 16 and 20 days of age is probably set off by the rise in the serum corticosterone level.