Expression of hybrid class I genes of the major histocompatibility complex in mouse L cells.
Expression of hybrid class I genes of the major histocompatibility complex in mouse L cells.
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小鼠 L 细胞中主要组织相容性复合物的杂合 I 类基因的表达。
DOI：
10.1073/pnas.82.18.6245
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发表时间：
1985
影响因子：
11.1
通讯作者：
Hood,L
中科院分区：
文献类型：
--
作者：
Straus,DS;Stroynowski,I;Schiffer,SG;Hood,L
小鼠主要组织相容性复合体的I类基因可分为两类：编码移植抗原的基因和编码Qa和Tla抗原的基因。近交 BALB/c 小鼠有 28 个潜在的 Qa/Tla 基因。这些基因的组织表达位点、发育调节和功能实际上是未知的。我们使用外显子改组技术在三个 Qa 区域基因（Q5、Q7 和 Q8）与另外两个 I 类基因（H-2Ld 和 Q6）之间构建杂合基因。杂合基因已被转染至小鼠L细胞中，其中通常表达完整的移植抗原基因并且其中通常不表达完整的Qa基因。对杂合基因构建体表达的分析表明，两个 Qa 基因（Q5 和 Q8）的 5' 半部可以在杂合分子的背景下容易地表达，而 3' 半部则阻止细胞表面表达。这里描述的外显子改组方法将有助于表征 Qa/Tla 基因以及鉴定或生产新试剂来研究 Qa/Tla 基因产物、它们的组织分布、它们的表达发育阶段以及最终的它们的功能。
The class I genes of the major histocompatibility complex of the mouse can be divided into two categories: those encoding the transplantation antigens and those encoding the Qa and Tla antigens. The inbred BALB/c mouse has 28 potential Qa/Tla genes. The sites of tissue expression, developmental regulation, and functions of these genes are virtually unknown. We have used the technique of exon shuffling to construct hybrid genes between each of three Qa region genes (Q5, Q7, and Q8) and two other class I genes (H-2Ld and Q6). The hybrid genes have been transfected into mouse L cells, in which intact transplantation antigen genes generally are expressed and in which intact Qa genes generally are not expressed. Analysis of expression of the hybrid gene constructs indicates that the 5' half of two of the Qa genes (Q5 and Q8) can readily be expressed in the context of a hybrid molecule, whereas the 3' half prevents cell-surface expression. The exon shuffling approach described here will be useful in characterizing Qa/Tla genes and in identifying or producing new reagents to study the Qa/Tla gene products, their tissue distribution, their developmental stages of expression, and, ultimately, their functions.