A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field
A detailed ethological analysis of the mouse open field test: effects of diazepam, chlordiazepoxide and an extremely low frequency pulsed magnetic field
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DOI：
10.1016/s0149-7634(01)00011-2
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发表时间：
2001-05-01
影响因子：
8.2
通讯作者：
Prato, FS
中科院分区：
文献类型：
--
作者：
Choleris, E;Thomas, AW;Prato, FS
开放式测试（FAST）是一个广泛使用的程序，用于检查药物和焦虑的行为影响。缺乏对动物行为的详细伦理评估。在这里，我们介绍了对苯二氮卓类药物，地西ep剂，地西ep（DZ，1.5 mg/kg）和氯氮卓氮杂氧化物（CDP，5.0和10.0mg/kg）的急性治疗影响的详细伦理评估。我们研究了各种行为的持续时间，频率和时间过程（即探索，步行，后，伸展运动，伸展，返回，坐着，坐着，旋转转弯，跳跃，跳跃和睡眠），在新型开放田的不同地区展示了雄性小鼠。 DZ和CDP始终降低了伸展运动的典型焦虑样行为和墙壁围墙（thigmotaxis），以及其他新措施的行为：“回报”，而不会对总体运动产生任何整体影响。这些药物的作用也有所不同。 CDP引起了THR运动模式的转变，从“高探索”到“高步道”，而DZ主要引起了Sit and Groom的改变。两次接触MAC处理，均减少了水平和垂直（饲养）活性，并增加了修饰和旋转转弯。然而。伸展运动的类似焦虑症的行为仅通过第一次接触而略微降低。我们得出的结论是，对OFT进行的详细伦理分析不仅允许检测药物和非药物剂（即脉冲磁场）对焦虑样行为的特定作用，而且还允许检查非特异性效应，尤其是对一般活性的影响。 （c）2001 Elsevier Science Ltd.保留权利。
The open field test (OFT) is a widely used procedure for examining the behavioral effects of drugs and anxiety. Detailed ethological assessments of animal behavior are lacking. Here we present a detailed ethological assessment of the effects of acute treatment with the benzodiazepines, diazepam (DZ, 1.5 mg/kg) and chlordiazepoxide (CDP, 5.0 and 10.0mg/kg), as well as exposure to a non-pharmacological agent, a specific pulsed extremely low frequency magnetic field (MAG) on open field behavior. We examined the duration, frequency and time course of various behaviors (i.e. exploration, walk, rear, stretch attend, return, groom, sit, spin turn, jump and sleep) exhibited by male mice in different regions of a novel open field. Both DZ and CDP consistently reduced the typical anxiety-like behaviors of stretch attend and wall-following (thigmotaxis), along with that of an additional new measure:'returns', without producing any overall effects on total locomotion. The drugs also differed in their effects. CDP elicited a shift in thr locomotor pattern from a 'high explore' to a 'high walk', while DZ mainly elicited alterations in sit and groom. The MAC treatment was repeated twice with both exposures reducing horizontal and vertical (rearing) activity and increasing grooming and spin turns. However. the anxiety-like behaviors of stretch attend and return were marginally reduced by only the first exposure. We conclude that a detailed ethological analysis of the OFT allows not only the detection of specific effects of drugs and non-pharmacological agents (i.e. pulsed magnetic field) on anxiety-like behaviors, but also permits the examination of non-specific effects, in particular those on general activity. (C) 2001 Elsevier Science Ltd. Ail rights reserved.