High-Pressure Carbon Monoxide and Oxygen Mixture is Effective for Lung Preservation
High-Pressure Carbon Monoxide and Oxygen Mixture is Effective for Lung Preservation
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DOI：
10.3390/ijms20112719
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发表时间：
2019-06-01
影响因子：
5.6
通讯作者：
Yokomise, Hiroyasu
中科院分区：
文献类型：
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作者：
Fujiwara, Atsushi;Hatayama, Naoyuki;Yokomise, Hiroyasu
(1)背景资料：血红素加氧酶-1（HO-1）降解血红素并产生一氧化碳（CO），产生各种抗炎、抗氧化和抗凋亡作用。本研究旨在通过高压气体（HPG）保存法，观察CO对供肺缺血再灌注损伤（IRI）的影响。(2)研究方法：供体大鼠和犬肺保存在充满CO（1.5 atm）和氧气（O-2; 2 atm）的室中，并在保存前立即用CO和O-2混合物（CO/O-2组）或空气（空气组）通气。大鼠肺进行异位颈部移植，并在再灌注后进行评价，而犬肺进行同种异体移植和评价。(3)结果：CO/O-2组肺泡出血明显轻于空气组。HO-1的mRNA表达水平在两组中保持不变;然而，CO/O-2组的炎症介质水平显著低于空气组。两组移植肺的氧合相当，但空气组的乳酸水平往往较高。(4)结论：HO-1/CO系统在HPG保存方法中能有效抑制IRI，保存供肺。
(1) Background: Heme oxygenase-1 (HO-1) degrades heme and generates carbon monoxide (CO), producing various anti-inflammatory, anti-oxidative, and anti-apoptotic effects. This study aimed to confirm the effects of CO on the ischemia-reperfusion injury (IRI) of donor lungs using a high-pressure gas (HPG) preservation method. (2) Methods: Donor rat and canine lungs were preserved in a chamber filled with CO (1.5 atm) and oxygen (O-2; 2 atm) and were ventilated with either CO and O-2 mixture (CO/O-2 group) or air (air group) immediately before storage. Rat lungs were subjected to heterotopic cervical transplantation and evaluated after reperfusion, whereas canine lungs were subjected to allogeneic transplantation and evaluated. (3) Results: Alveolar hemorrhage in the CO/O-2 group was significantly milder than that in the air group. mRNA expression levels of HO-1 remained unchanged in both the groups; however, inflammatory mediator levels were significantly lower in the CO/O-2 group than in the air group. The oxygenation of graft lungs was comparable between the two groups, but lactic acid level tended to be higher in the air group. (4) Conclusions: The HO-1/CO system in the HPG preservation method is effective in suppressing IRI and preserving donor lungs.