Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis In Vivo and In Vitro
Sulforaphane Improves Lipid Metabolism by Enhancing Mitochondrial Function and Biogenesis In Vivo and In Vitro
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DOI：
10.1002/mnfr.201800795
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发表时间：
2019-02-01
影响因子：
5.2
通讯作者：
Shan, Yujuan
中科院分区：
文献类型：
--
作者：
Lei, Peng;Tian, Sicong;Shan, Yujuan
据报道，萝卜硫素（SFN）可减少脂质的积累。然而，其背后的机制仍不清楚。在这项研究中，SFN通过改变线粒体功能和生物发生相关机制来改善脂质代谢的潜力。方法与结果采用高脂饲料喂养10周，建立HHL-5细胞和大鼠脂代谢异常模型。目前的研究结果表明，SFN使线粒体肿胀并刺激线粒体生物合成。SFN可逆转NRF 1和TFAM表达的降低。SFN通过核因子红细胞-2相关因子（Nrf 2）激活增加抗氧化化合物的水平。此外，SFN改善多种线粒体生物活性，如线粒体膜电位、ATP和基于PGC-1 α途径的电子传递链。SFN还通过转录上调脂肪甘油三酯脂肪酶（ATGL）和脂肪敏感性脂肪酶（HSL）来激活脂解。结论SFN通过PGC- 1 α依赖性促进线粒体生物合成和Nrf 2依赖性改善线粒体功能来增强脂质利用。
Scope Sulforaphane (SFN) is reported to reduce the accumulation of lipids. However, the underling mechanism remains unclear. In this study, the potential of SFN to improve lipid metabolism is investigated through altering mitochondrial function and biogenesis-related mechanisms. Methods and Results The abnormal lipid metabolism model was established both in HHL-5 cells and in rats by feeding a high-fat diet (HFD) for 10 weeks. The current findings suggest that SFN alleviates the swelling of mitochondria and stimulates mitochondrial biogenesis. The reduced expression of NRF1 and TFAM, were reversed by SFN. SFN increases the levels of antioxidant compounds via nuclear factor erythroid-2-related factor (Nrf2) activation. Furthermore, SFN improves multiple mitochondrial bioactivities, such as mitochondrial membrane potential, ATP, and the electron transfer chain based on PGC-1 alpha pathway. SFN also activates lipolysis by transcriptionally upregulating adipose triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL). Conclusions SFN enhances utilization of lipids via both the PGC- 1 alpha-dependent promotion of mitochondrial biogenesis and Nrf2 dependent improvement of mitochondrial function.