In vitro analysis of protection of the enzyme bile salt hydrolase against enteric conditions by whey protein-gum arabic microencapsulation
In vitro analysis of protection of the enzyme bile salt hydrolase against enteric conditions by whey protein-gum arabic microencapsulation
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DOI：
10.1021/jf801068u
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发表时间：
2008-09-24
影响因子：
6.1
通讯作者：
Kleerebezem, M.
中科院分区：
文献类型：
--
作者：
Lambert, J. M.;Weinbreck, F.;Kleerebezem, M.
对健康促进物质的有效肠道递送的兴趣正在增加。然而，需要特别注意易受伤害物质如酶的输送。通过pH非常低的胃的运输可能对待递送的蛋白质的酶活性有害。在这里，我们描述了微胶囊化的模型酶胆盐水解酶（BSH）使用乳清蛋白-阿拉伯树胶微胶囊食品级和有针对性的酶在小肠的近端区域的交付。此外，在体外将肠溶包衣微胶囊用于位点特异性酶递送的功效与内源性产生Bsh酶的活植物乳杆菌WCFS 1细菌进行比较。微胶囊允许在胃条件下的酶的高度有效的保护。此外，Bsh释放在肠道条件下似乎是非常有效的，虽然在胰酶的存在下，Bsh活性下降的时间，由于蛋白水解降解。相比之下，L. plantarum似乎能够承受胃的条件以及胰酶的挑战。使用胶囊和活细菌的递送各自具有所讨论的不同的（缺点）优点。总之，活细菌和食品级微胶囊提供了用于特定酶的专用肠递送的替代方案，并且待递送的酶的选择可以确定哪种递送模式是最合适的。
The interest in efficient intestinal delivery of health-promoting substances is increasing. However, the delivery of vulnerable substances such as enzymes requires specific attention. The transit through the stomach, where the pH is very low, can be detrimental to the enzymatic activity of the protein to be delivered. Here, we describe the microencapsulation of the model enzyme bile salt hydrolase (Bsh) using whey protein-gum arabic microencapsulates for food-grade and targeted enzyme delivery in the proximal region of the small intestine. Furthermore, the efficacy of enteric coating microencapsulates for site-specific enzyme delivery was compared in vitro with living Lactobacillus plantarum WCFS1 bacteria that endogenously produce the Bsh enzyme. Microencapsulates allowed highly effective protection of the enzyme under gastric conditions. Moreover, Bsh release under intestinal conditions appeared to be very efficient, although in the presence of pancreatin, the Bsh activity decreased in time due to proteolytic degradation. In comparison, L. plantarum appeared to be capable to withstand gastric conditions as well as pancreatin challenge. Delivery using encapsulates and live bacteria each have different (dis)advantages that are discussed. In conclusion, live bacteria and food-grade microencapsulates provide alternatives for dedicated enteric delivery of specific enzymes, and the choice of enzyme to be delivered may determine which mode of delivery is most suitable.