A bioenergetic model of gossypol action: effects of gossypol on adult rat spermatogenic cells.
A bioenergetic model of gossypol action: effects of gossypol on adult rat spermatogenic cells.
复制标题
棉酚作用的生物能模型：棉酚对成年大鼠生精细胞的影响。
DOI：
10.1152/ajpcell.1988.254.4.c564
复制
发表时间：
1988
期刊：
影响因子：
--
通讯作者：
Benos,DJ
中科院分区：
文献类型：
--
作者：
Reyes,J;Borriero,L;Benos,DJ
大鼠生精细胞的糖酵解率为 124 +/- 36 nmol 乳酸.mg 蛋白-1.h-1。在没有氧化磷酸化的情况下，最大糖酵解速率不会超过基础速率 40% 以上。从耗氧量和乳酸生成率可以计算出，生精细胞产生的总 ATP 中不到 8% 由糖酵解提供。生精细胞的这些特性使它们非常容易受到细胞毒性的影响，这是由于棉酚等物质的作用会损害线粒体氧化磷酸化，或者是产生睾丸缺氧的条件。棉酚对生精细胞能量代谢的主要作用是解偶联线粒体氧化磷酸化。在没有葡萄糖的情况下，棉酚的这种解偶联作用会改变 NAD+/NADH 氧化还原偶联，因此可以通过乳酸脱氢酶催化反应改变代谢通量。
Rat spermatogenic cells have glycolytic rates of 124 +/- 36 nmol lactate.mg protein-1.h-1. Maximum glycolytic rates in the absence of oxidative phosphorylation do not exceed the basal rates by greater than 40%. From the rates of oxygen consumption and lactate production it can be calculated that less than 8% of the total ATP produced by spermatogenic cells is provided by glycolysis. These properties of spermatogenic cells would render them highly susceptible to cytotoxicity due to the action of agents like gossypol that impair mitochondrial oxidative phosphorylation or to conditions that produce testicular hypoxia. The main effect of gossypol on spermatogenic cell energy metabolism is to uncouple mitochondrial oxidative phosphorylation. In the absence of glucose, this uncoupling action of gossypol would change the NAD+/NADH redox coupling and, consequently, could modify metabolic fluxes through the lactic dehydrogenase catalyzed reaction.