Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array
Liquid Chromatography Chip with Low-Dispersion and Low-Pressure-Drop Turn Structure Utilizing a Distribution-Controlled Pillar Array
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DOI：
10.1021/acs.analchem.6b01201
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发表时间：
2016-06-21
影响因子：
7.4
通讯作者：
Tsunoda, Makoto
中科院分区：
文献类型：
--
作者：
Isokawa, Muneld;Takatsuki, Katsuya;Tsunoda, Makoto
为了实现高效、快速的旋转柱阵列分离，开发了一种低分散、低压降的新型旋转柱阵列。这种“柱分布控制”（PDC）转弯被设计为填充有八边形柱的恒定半径转弯，所述八边形柱被布置成控制移动的相在径向方向上的线速度。在通过计算流体动力学分析调整柱位置之后，在20 X 20 mm（2）硅玻璃板上制造具有两个转弯的27 mm长的柱阵列柱。PDC转弯抑制样品分散的程度与先前开发的锥形转弯相似，并且新设计的转弯的压降降低到类似于锥形转弯的1/6。此外，C18修饰的柱阵列柱与PDC转向显示出良好的生物分析适用性;五个荧光标记的氨基酸，在7.5 mm/s的线速度仅24 s的分离。所开发的转弯结构提供了更长的柱阵列柱的优点，具有更多的转弯，用于复杂样品的快速分析。
To realize efficient, fast separations on pillar array columns with turns, a novel turn with low-dispersion and low-pressure-drop properties was developed. This "pillar-distribution controlled" (PDC) turn was designed as a constant-radius-turn filled with octagonal pillars that were arranged to control the linear velocity of the mobile phase in the radial direction. After the pillar positions were adjusted by computational fluid dynamics analysis, 27 mm long pillar array columns with two turns were fabricated on a 20 x 20 mm(2) silicon glass plate. The PDC turns suppressed the sample dispersion to a similar extent as the previously developed tapered turn, and the pressure drop of the newly designed turn was reduced to similar to 1/6 that of the tapered turn. Moreover, the C18-modified pillar array column with the PDC turns showed good bioanalytical applicability; five fluorescendy labeled amino acids were separated in only 24 s at a linear velocity of 7.5 mm/s. The developed turn structure offers the advantages of longer pillar array columns with more turns for the fast analysis of complex samples.