Size Dependency of Circulation and Biodistribution of Biomimetic Nanoparticles: Red Blood Cell Membrane-Coated Nanoparticles
Size Dependency of Circulation and Biodistribution of Biomimetic Nanoparticles: Red Blood Cell Membrane-Coated Nanoparticles
复制标题
仿生纳米颗粒的循环和生物分布的尺寸依赖性：红细胞膜包被的纳米颗粒
DOI：
10.3390/cells8080881
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发表时间：
2019-08-01
期刊：
影响因子：
6
通讯作者：
Pang, Zhiqing
中科院分区：
文献类型：
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作者：
Li, Haichun;Jin, Kai;Pang, Zhiqing
近年来，仿生纳米粒子，特别是细胞膜包裹纳米粒子，通过模仿生物系统的结构和功能，如在血液中的长循环寿命，在抗肿瘤治疗、解毒和免疫调节等生物医学应用中引起了越来越多的关注。然而，细胞膜包裹的纳米颗粒的循环时间远小于原始细胞，极大地限制了其生物医学应用，而其根本原因很少得到证明。本研究以红细胞膜包覆纳米粒（RBC-NPs）为模型仿生纳米粒，考察了粒径对RBC-NPs循环和生物分布的影响。通过将RBC膜融合在聚（乳酸-共-乙醇酸）纳米颗粒上来制备不同尺寸的RBC-NP（80、120、160和200 nm）。结果表明，粒径并没有改变这些仿生纳米颗粒的细胞摄取的巨噬细胞在体外和它们的免疫原性反应在体内。然而，它们在体内的循环寿命随着粒径的增加而减少，而它们在肝脏中的积累随着粒径的增加而增加，这可能与它们通过肝窦的尺寸依赖性过滤有关。这些发现将为仿生纳米粒子的设计和优化提供实验依据。
Recently, biomimetic nanoparticles, especially cell membrane-cloaked nanoparticles, have attracted increasing attention in biomedical applications, including antitumor therapy, detoxification, and immune modulation, by imitating the structure and the function of biological systems such as long circulation life in the blood. However, the circulation time of cell membrane-cloaked nanoparticles is far less than that of the original cells, greatly limiting their biomedical applications, while the underlying reasons are seldom demonstrated. In this study, the influence of particle size on the circulation and the biodistribution of red blood cell membrane-coated nanoparticles (RBC-NPs) as model biomimetic nanoparticles were investigated. Differently sized RBC-NPs (80, 120, 160, and 200 nm) were prepared by fusing RBC membranes on poly(lactic-co-glycolic acid) nanoparticles. It was shown that the particle size did not change the cellular uptake of these biomimetic nanoparticles by macrophage cells in vitro and their immunogenic responses in vivo. However, their circulation life in vivo decreased with the particle size, while their accumulation in the liver increased with the particle size, which might be related to their size-dependent filtration through hepatic sinusoids. These findings will provide experimental evidence for the design and the optimization of biomimetic nanoparticles.