Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots.
Enhanced Specificity of Multiplex Polymerase Chain Reaction via CdTe Quantum Dots.
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DOI：
10.1007/s11671-010-9797-5
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发表时间：
2011-12
影响因子：
--
通讯作者：
Xiao, Zhongdang
中科院分区：
文献类型：
--
作者：
Liang, Gaofeng;Ma, Chao;Zhu, Yanliang;Li, Shuchun;Shao, Youhua;Wang, Yong;Xiao, Zhongdang
最近报道纳米颗粒能够提高聚合酶链式反应（PCR）的效率和特异性。在这里，将CdTe量子点引入多重PCR系统。结果表明，适当浓度的CdTe量子点可以通过减少复合体系中非特异性产物的形成来提高多重PCR的性能，但过量的CdTe量子点会抑制PCR。增加聚合酶浓度或加入牛血清白蛋白（BSA）可逆转量子点对PCR的影响。并对这些作用的机制进行了讨论。结果表明，CdTe量子点可用于PCR扩增产物的优化，特别是在不同引物退火温度的多重PCR体系中，对分子诊断具有重要意义。
Nanoparticles were recently reported to be able to improve both efficiency and specificity in polymerase chain reaction (PCR). Here, CdTe QDs were introduced into multi-PCR systems. It was found that an appropriate concentration of CdTe QDs could enhance the performance of multi-PCR by reducing the formation of nonspecific products in the complex system, but an excessive amount of CdTe QDs could suppress the PCR. The effects of QDs on PCR can be reversed by increasing the polymerase concentration or by adding bovine serum albumin (BSA). The mechanisms underlying these effects were also discussed. The results indicated that CdTe QDs could be used to optimize the amplification products of the PCR, especially in the multi-PCR system with different primers annealing temperatures, which is of great significance for molecular diagnosis.
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