HYDROGEN TURNOVER BY PSYCHROTROPHIC HOMOACETOGENIC AND MESOPHILIC METHANOGENIC BACTERIA IN ANOXIC PADDY SOIL AND LAKE SEDIMENT
HYDROGEN TURNOVER BY PSYCHROTROPHIC HOMOACETOGENIC AND MESOPHILIC METHANOGENIC BACTERIA IN ANOXIC PADDY SOIL AND LAKE SEDIMENT
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DOI：
10.1111/j.1574-6968.1989.tb03382.x
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发表时间：
1989-08-01
影响因子：
4.2
通讯作者：
SCHUTZ, H
中科院分区：
文献类型：
--
作者：
CONRAD, R;BAK, F;SCHUTZ, H
在康斯坦茨湖缺氧水稻土和沉积物中，研究了温度对甲烷的产生、溶解氢的周转和利用氢的厌氧菌的富集的影响。缺氧水稻土在H_2/CO_2气氛下培养，在高于25℃的温度下，甲烷的生成速率随时间的延长而增加。C，但在低于20℃时有所下降。氯仿可完全抑制缺氧水稻土和湖泊沉积物中甲烷的生成，但不能或仅部分抑制溶解氢的周转，尤其是在低温下。当培养温度低于20℃时，以H_2为能源的培养可使化石型同产乙酸菌得到丰富。C.亲水产甲烷菌只有在30℃时才能被浓缩。C来自缺氧水稻土。从4度湖泊沉积物富集培养中分离到一株高乙酸菌(HP4)和一株产甲烷菌(BAB1)。C和30℃的缺氧水稻土。C。这两个菌株在基本生长温度上有很大差异。产甲烷菌是中嗜性的，而高乙酸菌是嗜冷性的。这种适应可能允许高产乙酸菌在缺氧土壤和湖泊沉积物的低原位温度下促进溶解的H2的周转。
The effect of temperature on CH4 production, turnover of dissolved H2, and enrichment of H2-utilizing anaerobic bacteria was studied in anoxic paddy soil and sediment of Lake Constance. When anoxic paddy soil was incubated under an atmosphere of H2/CO2, rates of CH4 production increased with time at temperatures higher than 25.degree. C, but decreased at temperatures lower than 20.degree. C. Chloroform completely inhibited methanogenesis in anoxic paddy soil and lake sediment, but did not or only partially inhibit the turnover of dissolved H2, especially at low incubation temperatures. Cultures with H2 as energy source resulted in the enrichment of chemolithotrophic homoacetogenic bacteria whenever incubation temperatures were lower than 20.degree. C. Hydrogenotropic methanogens could only be enriched at 30.degree. C from anoxic paddy soil. A homoacetogen (strain HP4) and a methanogen (strain Bab1) were isolated from enrichment cultures with lake sediment at 4.degree. C and with anoxic paddy soil at 30.degree. C, respectively. The two strains greatly differed in cardinal temperatures of growth. Whereas the methanogen was mesophilic, the homoacetogen was psychrotrophic. This adaptation possibly allows homoacetogenic bacteria to contribute to the turnover of dissolved H2 at low in situ temperatures of anoxic soils and lake sediments.