Size-dependent positioning of human chromosomes in interphase nuclei
Size-dependent positioning of human chromosomes in interphase nuclei
复制标题
DOI：
10.1016/s0006-3495(00)76282-5
复制
发表时间：
2000-07-01
影响因子：
3.4
通讯作者：
Yokota, H
中科院分区：
文献类型：
--
作者：
Bin Sun, H;Shen, J;Yokota, H
通过使用荧光原位杂交技术，我们发现，9个不同的q臂端粒标记的染色体在人类G（1）间期细胞核中的定位是染色体大小依赖性的。大染色体的q臂端粒比小染色体的端粒更位于外周。通过确定杂交位点的x和y坐标并计算到人成纤维细胞核中心的均方根径向距离，发现了人细胞核内染色质的这种高度组织化的排列。我们在这里证明，全球组织内的G（1）间期核是受影响的一个最基本的物理量-染色体的大小或质量，并提出两个生物物理模型，体积排除模型和有丝分裂预设模型，来解释我们的发现。
By using a fluorescence in situ hybridization technique we revealed that for nine different q-arm telomere markers the positioning of chromosomes in human G(1) interphase nuclei was chromosome size-dependent. The q-arm telomeres of large chromosomes are more peripherally located than telomeres on small chromosomes. This highly organized arrangement of chromatin within the human nucleus was discovered by determining the x and y coordinates of the hybridization sites and calculating the root-mean-square radial distance to the nuclear centers in human fibroblasts. We demonstrate here that global organization within the G(1) interphase nucleus is affected by one of the most fundamental physical quantities-chromosome size or mass-and propose two biophysical models, a volume exclusion model and a mitotic preset model, to explain our finding.