Sialic acid‐dependent interaction of group B streptococci with influenza virus‐infected cells reveals a novel adherence and invasion mechanism
Sialic acid‐dependent interaction of group B streptococci with influenza virus‐infected cells reveals a novel adherence and invasion mechanism
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B 族链球菌与流感病毒感染细胞的唾液酸依赖性相互作用揭示了一种新的粘附和侵袭机制
DOI：
10.1111/cmi.12818
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发表时间：
2018
影响因子：
3.4
通讯作者：
Herrler G
中科院分区：
文献类型：
--
作者：
Tong J;Rohde M;Meng F;Valentin-Weigand P;Herrler G
B组链球菌(GBS)含有一种衣壳多糖，其侧链末端为α-2，3-连接的唾液酸。由于这种连锁类型，GBS的唾液酸被免疫细胞的凝集素识别。这种相互作用会抑制宿主的免疫反应，从而促进免疫逃避。由于几种甲型流感病毒使用α2，3-连接唾液酸作为结合宿主细胞的受体决定因素，我们分析了GBS和流感病毒是否可以相互作用，以及这种相互作用如何影响病毒复制和细菌对宿主细胞的黏附和入侵。共沉淀试验表明，偏爱α2，3-连接唾液酸的病毒以唾液酸依赖的方式与GBS结合。然而，血凝抑制试验表明，不同亚型禽流感病毒之间的结合效率存在很大差异。IAV生长曲线的延迟表明GBS对病毒复制有抑制作用。另一方面，当细胞预先感染具有适当受体特异性的IAV时，GBS的黏附和侵袭效率都得到了提高。我们的结果表明，当患者同时感染流感病毒时，GBS感染可能会导致更严重的疾病。这种联合感染机制可能也与其他人类疾病有关，因为有更多的细菌病原体与α2，3-连结的唾液酸和人类病毒结合到这种连锁型。
Group B streptococci (GBS) contain a capsular polysaccharide with side chains terminating in α2,3‐linked sialic acids. Because of this linkage type, the sialic acids of GBS are recognised by lectins of immune cells. This interaction results in a dampening of the host immune response and thus promotes immune evasion. As several influenza A viruses (IAV) use α2,3‐linked sialic acid as a receptor determinant for binding to host cells, we analysed whether GBS and influenza viruses can interact with each other and how this interaction affects viral replication and bacterial adherence to and invasion of host cells. A co‐sedimentation assay revealed that viruses with a preference for α2,3‐linked sialic acids bind to GBS in a sialic acid‐dependent manner. There is, however, a large variation in the efficiency of binding among avian influenza viruses of different subtypes as shown by a hemagglutination‐inhibition assay. A delay in the growth curve of IAV indicated that GBS has an inhibitory effect on virus replication. On the other hand, both the adherence and invasion efficiency of GBS were enhanced when the cells were pre‐infected by IAV with appropriate receptor specificity. Our results suggest that GBS infection may result in a more severe disease when patients are co‐infected by influenza viruses. This co‐infection mechanism may have relevance also to other human diseases, as there are more bacterial pathogens with α2,3‐linked sialic acids and human viruses binding to this linkage type.
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影响因子：
30.3
作者：
Siegel SJ;Roche AM;Weiser JN
通讯作者：
Weiser JN
影响因子：
7.3
作者：
Landwehr-Kenzel S;Henneke P
通讯作者：
Henneke P
影响因子：
4.6
作者：
Meng F;Wu NH;Seitz M;Herrler G;Valentin-Weigand P
通讯作者：
Valentin-Weigand P
DOI：
10.1073/pnas.1414422112
发表时间：
2015-01-06
影响因子：
11.1
作者：
Li, Ning;Ren, Aihui;Wang, Beinan
通讯作者：
Wang, Beinan
影响因子：
4.6
作者：
Wu NH;Yang W;Beineke A;Dijkman R;Matrosovich M;Baumgärtner W;Thiel V;Valentin-Weigand P;Meng F;Herrler G
通讯作者：
Herrler G