Thiamin biosynthesis in eukaryotes:: Characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis
Thiamin biosynthesis in eukaryotes:: Characterization of the enzyme-bound product of thiazole synthase from Saccharomyces cerevisiae and its implications in thiazole biosynthesis
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DOI：
10.1021/ja061413o
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发表时间：
2006-06-07
影响因子：
15
通讯作者：
Begley, Tadhg P.
中科院分区：
文献类型：
--
作者：
Chatterjee, Abhishek;Jurgenson, Christopher T.;Begley, Tadhg P.
焦磷酸硫胺素在真核生物中的生物合成不同于原核生物的生物合成，迄今为止知之甚少。只有一个噻唑生物合成基因已被确定（Thi 4在酿酒酵母）。在这里，我们报告的鉴定和表征的Thi 4结合的代谢产物，由ADP加合物的5-（2-羟乙基）-4-甲基噻唑-2-羧酸。该化合物的意外结构首次揭示了真核生物中硫胺素噻唑生物合成的机制。
The biosynthesis of thiamin pyrophosphate in eukaryotes is different from the prokaryotic biosynthesis and is poorly understood to date. Only one thiazole biosynthetic gene has been identified (Thi4 inSaccharomyces cerevisiae). Here we report the identification and characterization of a Thi4-bound metabolite that consists of the ADP adduct of 5-(2-hydroxyethyl)-4-methylthiazole-2-carboxylic acid. The unexpected structure of this compound yields the first insights into the mechanism of thiamin thiazole biosynthesis in eukaryotes.