MRC-5, human embryonic lung fibroblasts, induce the duplication of the developing chick limb bud.
MRC-5, human embryonic lung fibroblasts, induce the duplication of the developing chick limb bud.
复制标题
MRC-5，人胚胎肺成纤维细胞，诱导发育中的小鸡肢芽的复制。
DOI：
10.1006/dbio.1993.1302
复制
发表时间：
1993
影响因子：
2.7
通讯作者：
H. Ide
中科院分区：
文献类型：
--
作者：
S. Yonei;K. Tamura;E. Koyama;T. Nohno;S. Noji;H. Ide
雏鸡肢体的前后（A-P）轴模式可能是由肢芽后缘（ZPA，极化活动区）的一小部分间充质细胞控制的。在这项研究中，我们发现，当移植到肢芽的前部区域时，MRC-5 成纤维细胞具有沿着 A-P 轴形成鸡肢重复模式的能力。 MRC-5细胞仅在前肢芽阶段有效，并且腿芽比翼芽反应更灵敏。当肢体发育进行时，移植细胞保留在肢芽的基部。这些结果表明，MRC-5细胞的产物参与了重复图案形成的三个可能的过程：诱导极化活性；维持这种活动，这种活动在前肢芽阶段表现较弱；以及确定 A-P 轴作为 ZPA 因子。通过允许使用少量具有极化活性的胚胎组织细胞，对 MRC-5 细胞系重复形成的分析为阐明 ZPA 的分子性质提供了强大的工具。
The anteroposterior (A-P) axis pattern of the chick limb is likely to be controlled by a small region of mesenchyme cells at the posterior margin of the limb bud (ZPA, zone of polarizing activity). In this study, we found that MRC-5 fibroblast cells had the capacity for duplicated-pattern formation of the chick limb along the A-P axis when grafted to the anterior region of the limb bud. MRC-5 cells were effective only during pre-limb bud stages, and the leg bud was more responsive than the wing bud. Grafted cells remained at the base of the limb bud when limb development proceeded. These results suggest that the products of MRC-5 cells are involved in three possible processes of duplicated-pattern formation: induction of the polarizing activity; maintenance of this activity, which is present weakly at pre-limb bud stages; and determination of A-P axis as the ZPA factor(s). By allowing the use of a small number of cells of embryonic tissues with polarizing activity, the analysis of duplicate formation with the MRC-5 cell line provides a powerful tool for elucidating the molecular nature of the ZPA.