Reduction of endogenous transforming growth factors β prevents ontogenetic neuron death
Reduction of endogenous transforming growth factors β prevents ontogenetic neuron death
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DOI：
10.1038/80598
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发表时间：
2000-11-01
影响因子：
25
通讯作者：
Unsicker, K
中科院分区：
文献类型：
--
作者：
Krieglstein, K;Richter, S;Unsicker, K
我们发现，免疫中性化的内源性转化生长因子β（TGF-β）在鸡胚，个体发育神经元死亡的睫状神经元，背根和脊髓运动神经元在很大程度上防止，和神经元损失后，肢芽消融大大减少。同样地，在睫状神经节中个体发生细胞死亡期间，通过用T β R-II融合蛋白治疗来阻止TGF-β信号传导拯救了所有正常死亡的神经元。TUNEL染色显示抗体处理后凋亡细胞数量减少。外源性TGF-β挽救了TGF-β剥夺表型。我们的结论是，TGF-β是至关重要的调节个体发育神经元死亡以及神经元靶剥夺后的细胞死亡。
We show that following immunoneutralization of endogenous transforming growth factors beta (TGF-beta) in the chick embryo, ontogenetic neuron death of ciliary, dorsal root and spinal motor neurons was largely prevented, and neuron losses following limb bud ablation were greatly reduced. Likewise, preventing TGF-beta signaling by treatment with a T betaR-II fusion protein during the period of ontogenetic cell death in the ciliary ganglion rescued all neurons that normally die. TUNEL staining revealed decreased numbers of apoptotic cells following antibody treatment. Exogenous TGF-beta rescued the TGF-beta -deprived phenotype. We conclude that TGF-beta is critical in regulating ontogenetic neuron death as well as cell death following neuronal target deprivation.