Facile microfluidic device for droplets emulsion from small samples
Facile microfluidic device for droplets emulsion from small samples
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DOI：
10.1007/s41981-022-00229-x
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发表时间：
2022-11
影响因子：
2.7
通讯作者：
Dongliang He;Zerui Wu;Yao Lin;Guoyuan Li;Zhanhao Zhang;Yuheng Qiu;Yanan Ma;Chengqi Jia;Shangyu Wei;Guangzhu Shang;Xingyue Lei;P. Hu;Liqun He
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文献类型：
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作者：
Dongliang He;Zerui Wu;Yao Lin;Guoyuan Li;Zhanhao Zhang;Yuheng Qiu;Yanan Ma;Chengqi Jia;Shangyu Wei;Guangzhu Shang;Xingyue Lei;P. Hu;Liqun He
我们提出了一种用于制备单分散微滴的微流控装置，具有小体积的样品或试剂，这是免功耗，用户友好，仅基于一个高度便携式注射器。我们的方法只需要手动操作的注射器和尖锐的毛细管，没有任何复杂的实验设置，使整个系统的便携式和用户友好的非技术用户。通过在现场拉动注射器的柱塞来产生真空，并且通过真空产生流体。液滴尺寸可以通过调节微孔尺寸和毛细管或真空度来灵活控制。更有可能的是，微升样品或试剂也可以由其制备成乳液。与传统方法不同，我们的设备是自激活的，无需外部电源，同时确保更少的样品或试剂消耗，简化操作并节省成本，便于即时诊断测试或现场痕量物质检测。
We presented a microfluidic device for the preparation of monodisperse microdroplets with a small volume of samples or reagents, which is power-free, user-friendly and based only on a highly portable syringe. Our method requires only hand-operated syringes and sharpened capillaries without any complicated experimental setups, making the entire system portable and user-friendly for non-technical users. Vacuum is generated by pulling the plunger of the syringe on site, and fluid is generated by vacuum. The droplet size can be flexibly controlled by adjusting the size of micropores and capillary or vacuum degree. More potentially, microliter samples or reagents can also be prepared from it into emulsions. Unlike traditional methods, our device is self-activated, avoiding the need for external power supply while ensuring fewer samples or reagents consumption, simplifying operations and saving costs, facilitating immediate diagnostic testing or field trace substance detection.