Unbiased stereological estimation of the total number of synapses in a brain region
Unbiased stereological estimation of the total number of synapses in a brain region
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DOI：
10.1007/bf02284843
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发表时间：
1996-12-01
期刊：
影响因子：
--
通讯作者：
West, MJ
中科院分区：
文献类型：
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作者：
Geinisman, Y;Gundersen, HJG;West, MJ
现代体视学方法已被用来对 5 只兔子海马 CA1 区放射纹状体突触总数进行无偏估计。所使用的方法涉及两阶段分析，通常适用于神经系统的所有部分。在分析的第一阶段，根据卡瓦列里原理，在光学显微镜水平上通过点计数来估计参考体积。在第二阶段，突触的数值密度是在电子显微镜水平上用解剖器估计的。突触总数计算为数值密度和区域体积的乘积。在整个 CA1 区域的所有三个维度上进行点和解剖器采样，确保该区域的所有部分及其内的所有突触具有相同的采样概率。根据不同采样级别（即点、部分、个体动物和动物组的级别）的体积和突触数值密度的方差，对突触总数估计的精度进行了分析。提供了用于估计突触总数、评估估计精度和优化采样方案的程序的详细描述。
Modern stereological methods have been used to make unbiased estimates of the total number of synapses in the striatum radiatum of the hippocampal CA1 region of five rabbits. The approach used involved a two stage analysis and is generally applicable to all parts of the nervous system. During the first stage of the analysis, the reference volume was estimated by point counting, at the light microscope level, according to the Cavalieri principle. During the second stage, the numerical density of synapses was estimated with disectors at the electron microscopic level. The total number of synapses was calculated as the product of the numerical density and the volume of the region. The sampling with points and disectors was carried out in all three dimensions of the entire CA1 region in a manner that ensured that all parts of the region and all synapses within it had equal probabilities of being sampled. An analysis of the precision of the estimate of total synapse number has been performed in terms of the variances of volume and synaptic numerical density at different levels of sampling, i.e. at the level of points, sections, individual animals and group of animals. Detailed descriptions of the procedures used to estimate the total number of synapses, evaluate the precision of the estimates, and optimize the sampling scheme are provided.