Responses of Peripheral Blood Flow to Acute Hypoxia and Hyperoxia as Measured by Optical Microangiography
Responses of Peripheral Blood Flow to Acute Hypoxia and Hyperoxia as Measured by Optical Microangiography
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DOI：
10.1371/journal.pone.0026802
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发表时间：
2011-10-25
期刊：
影响因子：
3.7
通讯作者：
Wang, Ruikang K.
中科院分区：
文献类型：
--
作者：
Jia, Yali;Li, Peng;Wang, Ruikang K.
氧利用率被认为是代谢调节全身血流的关键因素。关于外周血流量（PBF）在缺氧和高氧期间如何受到影响和调节存在争议;然而，在氧相关生理扰动下对功能性PBF的体内评价仍然具有挑战性。显微镜观察，目前经常使用的成像模式PBF表征往往涉及使用外源性造影剂，这将不可避免地干扰微循环的内在生理反应正在研究。在本文中，光学微血管造影术（OMAG），使用固有的光学散射信号从血流反向散射成像PBF在骨骼肌挑战的氧浓度的变化。通过利用光学反射信号，我们证明OMAG能够显示血液动力学活动对急性缺氧和高氧的反应，包括调节大血管口径，微血管密度和不同大小的血管内骨骼肌在体内的流量调节。我们的研究结果表明，OMAG是一个很有前途的工具，在体内监测功能的大血管或微血管反应内外周血管床。
Oxygen availability is regarded as a critical factor to metabolically regulate systemic blood flow. There is a debate as to how peripheral blood flow (PBF) is affected and modulated during hypoxia and hyperoxia; however in vivo evaluating of functional PBF under oxygen-related physiological perturbation remains challenging. Microscopic observation, the current frequently used imaging modality for PBF characterization often involves the use of exogenous contrast agents, which would inevitably perturb the intrinsic physiologic responses of microcirculation being investigated. In this paper, optical micro-angiography (OMAG) was employed that uses intrinsic optical scattering signals backscattered from blood flows for imaging PBF in skeletal muscle challenged by the alteration of oxygen concentration. By utilizing optical reflectance signals, we demonstrated that OMAG is able to show the response of hemodynamic activities upon acute hypoxia and hyperoxia, including the modulation of macrovascular caliber, microvascular density, and flux regulation within different sized vessels within skeletal muscle in mice in vivo. Our results suggest that OMAG is a promising tool for in vivo monitoring of functional macro-or micro-vascular responses within peripheral vascular beds.