Comparative quantitative trait loci for silique length and seed weight in Brassica napus.
Comparative quantitative trait loci for silique length and seed weight in Brassica napus.
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甘蓝型油菜长角果长度和种子重量数量性状位点的比较
DOI：
10.1038/srep14407
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发表时间：
2015-09-23
影响因子：
4.6
通讯作者：
Qian W
中科院分区：
文献类型：
--
作者：
Fu Y;Wei D;Dong H;He Y;Cui Y;Mei J;Wan H;Li J;Snowdon R;Friedt W;Li X;Qian W
角果长和粒重是甘蓝型油菜重要的产量相关性状。虽然在B中已经鉴定出了许多SL和SW的数量性状基因座（QTL）。napus，很少对这些QTL进行比较分析。本研究在油菜双单倍体（DH）和DH衍生的重建F2群体中分别鉴定了20个和21个SL和SW QTL，分别解释了3年内表型变异的55.1%-74.3%和24.4%-62.9%。通过与芸苔属作物参考基因组的QTL置信区间比对，发现A09染色体上的17个QTL与C 08染色体上的2个QTL具有部分或完全重叠的置信区间。通过对极端表型DH系的高密度选择性基因分型，利用芸苔属单核苷酸多态性（SNP）芯片，将A09染色体上的QTL缩小并排列到B染色体R 09上30.84 ~ 31.98 MB的1.14-MB区域。在B的A09号染色体上27.21 ~ 28.26 MB的1.05-MB区。油菜。与芸苔属参考基因组的QTL比对显示，SL的QTL在A09和C 08上具有同源性。缩小的QTL区域为基因克隆和分子标记辅助育种提供了线索。
Silique length (SL) and seed weight (SW) are important yield-associated traits in rapeseed (Brassica napus). Although many quantitative trait loci (QTL) for SL and SW have been identified in B. napus, comparative analysis for those QTL is seldom performed. In the present study, 20 and 21 QTL for SL and SW were identified in doubled haploid (DH) and DH-derived reconstructed F2 populations in rapeseed, explaining 55.1–74.3% and 24.4–62.9% of the phenotypic variation across three years, respectively. Of which, 17 QTL with partially or completely overlapped confidence interval on chromosome A09, were homologous with two overlapped QTL on chromosome C08 by aligning QTL confidence intervals with the reference genomes of Brassica crops. By high density selective genotyping of DH lines with extreme phenotypes, using a Brassica single-nucleotide polymorphism (SNP) array, the QTL on chromosome A09 was narrowed and aligned into 1.14-Mb region from 30.84 to 31.98 Mb on chromosome R09 of B. rapa and 1.05-Mb region from 27.21 to 28.26 Mb on chromosome A09 of B. napus. The alignment of QTL with Brassica reference genomes revealed homologous QTL on A09 and C08 for SL. The narrowed QTL region provides clues for gene cloning and breeding cultivars by marker-assisted selection.