Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast
Spindle pole body movement is affected by glucose and ammonium chloride in fission yeast
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DOI：
10.1016/j.bbrc.2019.02.128
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发表时间：
2019-04-16
影响因子：
3.1
通讯作者：
Ueno, Masaru
中科院分区：
文献类型：
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作者：
Ito, Hiroaki;Sugawara, Takeshi;Ueno, Masaru
染色质动力学的复杂性是由几个活跃的过程协调的。在分裂酵母中，着丝粒聚集在纺锤体极体（SPB）周围，并以微管和三磷酸腺苷（ATP）依赖的方式振荡。然而，SPB振荡是否以及如何受到不同环境条件的影响仍然知之甚少。在这项研究中，我们定量运动的SPB组件，共定位与裂殖酵母中的着丝粒。我们发现，SPB运动显着减少在低葡萄糖浓度。SPB的运动也受到氯化铵存在的影响。功率谱分析表明，SPB的周期性运动被低葡萄糖浓度破坏。通过定量单细胞成像测量活细胞中的ATP水平表明，ATP水平不是SPB运动的唯一决定因素。我们的研究结果提供了新的见解SPB运动是如何调节细胞的能量状态和其他因素，如培养基的营养成分。(C)2019爱思唯尔公司All rights reserved.
The complexity of chromatin dynamics is orchestrated by several active processes. In fission yeast, the centromeres are clustered around the spindle pole body (SPB) and oscillate in a microtubule- and adenosine triphosphate (ATP)-dependent manner. However, whether and how SPB oscillation are affected by different environmental conditions remain poorly understood. In this study, we quantitated movements of the SPB component, which colocalizes with the centromere in fission yeast. We found that SPB movement was significantly reduced at low glucose concentrations. Movement of the SPB was also affected by the presence of ammonium chloride. Power spectral analysis revealed that periodic movement of the SPB is disrupted by low glucose concentrations. Measurement of ATP levels in living cells by quantitative single-cell imaging suggests that ATP levels are not the only determinant of SPB movement. Our results provide novel insight into how SPB movement is regulated by cellular energy status and additional factors such as the medium nutritional composition. (C) 2019 Elsevier Inc. All rights reserved.