A comparison study on microwave-assisted extraction of Artemisia sphaerocephala polysaccharides with conventional method: Molecule structure and antioxidant activities evaluation

A comparison study on microwave-assisted extraction of Artemisia sphaerocephala polysaccharides with conventional method: Molecule structure and antioxidant activities evaluation
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微波辅助提取白蒿多糖与常规方法的比较研究:分子结构及抗氧化活性评价

DOI:
10.1016/j.ijbiomac.2009.09.004
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发表时间:
2009-12-01
影响因子:
8.2
通讯作者:
Yao, Jian
Yao, Jian
中科院分区:
化学1区
文献类型:
--
作者:
Wang, Junlong;Zhang, Ji;Yao, Jian

文献摘要

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传统的多糖提取方法费时、费力、耗能大。采用微波辅助提取法对传统食品沙蒿多糖进行提取。采用Box-Behnken试验设计优化提取工艺。在微波加热过程中,在SEC-LLS测量中检测到分子量(M-w)降低。d(f)值为2.85,表明使用MAE的ASP在水溶液中表现为支链簇的球形构象。此外,与热水提取相比,其具有更强的抗氧化活性。结果表明,分子量对抗氧化活性影响较大。皇冠版权所有(C)2009年出版的爱思唯尔B. V.保留所有权利。
The conventional extraction methods for polysaccharides were time-consuming, laborious and energy-consuming. Microwave-assisted extraction (MAE) technique was employed for the extraction of Artemisia sphaerocephala polysaccharides (ASP),which is a traditional Chinese food. The extracting parameters were optimized by Box-Behnken design. In microwave heating process, a decrease in molecular weight (M-w) was detected in SEC-LLS measurement. A d(f) value of 2.85 indicated ASP using MAE exhibited as a sphere conformation of branched clusters in aqueous solution. Furthermore, it showed stronger antioxidant activities compared with hot water extraction. The data obtained showed that the molecular weights played a more important role in antioxidant activities. Crown Copyright (C) 2009 Published by Elsevier B.V. All rights reserved.