Correlation Between the Expression of MicroRNA-301a-3p and the Proportion of Th17 Cells in Patients with Rheumatoid Arthritis
Correlation Between the Expression of MicroRNA-301a-3p and the Proportion of Th17 Cells in Patients with Rheumatoid Arthritis
复制标题
类风湿关节炎患者MicroRNA-301a-3p表达与Th17细胞比例的相关性
DOI：
10.1007/s10753-016-0304-8
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发表时间：
2016-04-01
期刊：
影响因子：
5.1
通讯作者：
Wang, Shengjun
中科院分区：
文献类型：
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作者：
Tang, Xinyi;Yin, Kai;Wang, Shengjun
类风湿性关节炎（RA）的特征是慢性滑膜炎症和随后的关节破坏。既往研究已证实Th17细胞在RA发病机制中发挥着关键作用。 MicroRNA (miR)-301a-3p 是 Th17 细胞分化的调节因子，有助于自身免疫性疾病的发病机制。本研究的目的是鉴定RA患者中Th17细胞的改变并分析miR-301a-3p的表达与Th17细胞比例之间的相关性。结果显示，RA患者外周血单个核细胞（PBMC）中Th17细胞的频率和转录因子（RORγt和STAT3）的表达显着增加，相关的促炎细胞因子也上调。我们还观察到，活化STAT3蛋白抑制剂（PIAS3）（STAT3的主要细胞抑制剂）的表达在RA患者中减弱，并且与RA中Th17细胞的百分比呈负相关。有趣的是，PIAS3 表达抑制剂 miR-301a-3p 在 RA 患者的 PBMC 中过度表达，并且与 RA 患者中 Th17 细胞的频率呈正相关。综上所述，这些数据表明 miR-301a-3p 和 Th17 细胞在外周血中增加，这可能在 RA 的过程中发挥重要作用。
Rheumatoid arthritis (RA) is characterized by chronic synovial inflammation and subsequent joint destruction. Previous studies have confirmed that Th17 cells play a critical role in the pathogenesis of RA. MicroRNA (miR)-301a-3p is a regulatory factor for Th17 cells differentiation that contributes to the pathogenesis of autoimmune diseases. The purposes of this study were to identify the alteration of Th17 cells and analyze the correlation between the expression of the miR-301a-3p and the proportion of Th17 cells in RA patients. The results showed that the frequency of Th17 cells and the expression of transcription factors (RORγt and STAT3) significantly increased in the peripheral blood mononuclear cells (PBMCs) from RA patients, and the associated proinflammatory cytokines were also upregulated. We also observed that the expression of protein inhibitor of activated STAT3 (PIAS3), the main cellular inhibitor of STAT3, was attenuated in RA patients and negatively correlated with the percentage of Th17 cells in RA. Interestingly, miR-301a-3p, an inhibitor of PIAS3 expression, was overexpressed in the PBMCs from RA patients and positively correlated with the frequency of Th17 cells in patients with RA. Taken together, these data indicated that miR-301a-3p and Th17 cells were augmented in peripheral blood, which may play an important role in the process of RA.