Chronic hepatitis in chimpanzee carriers of hepatitis B virus: morphologic, immunologic, and viral DNA studies.
Chronic hepatitis in chimpanzee carriers of hepatitis B virus: morphologic, immunologic, and viral DNA studies.
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乙型肝炎病毒黑猩猩携带者的慢性肝炎：形态学、免疫学和病毒 DNA 研究。
DOI：
10.1073/pnas.77.10.6147
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发表时间：
1980
影响因子：
11.1
通讯作者：
Shafritz,DA
中科院分区：
文献类型：
--
作者：
Shouval,D;Chakraborty,PR;Ruiz-Opazo,N;Baum,S;Spigland,I;Muchmore,E;Gerber,MA;Thung,SN;Popper,H;Shafritz,DA
黑猩猩在感染乙型肝炎病毒数年后，可能表现出与人类相同的携带者状态。除了持续病毒感染的血清学证据外，经皮肝活检标本显示细胞质中有乙型肝炎病毒表面抗原，细胞核中有乙型肝炎病毒核心抗原。在人类慢性持续性肝炎中，有4只携带者有门静脉炎反应。通过分子杂交，克隆了含有乙型肝炎病毒DNA序列的血浆pA01，在肝活检标本的核酸提取物中证实了病毒DNA。虽然发现了一个长度大于假定病毒的病毒分子，但它似乎并不代表病毒DNA整合到宿主基因组中。黑猩猩模型可以作为研究乙型肝炎病毒持续存在和发展为慢性肝病机制的一种手段。
Years after infection with hepatitis B virus, chimpanzees may have manifestations of the carrier state as described in man. In addition to serologic evidence for persistent viral infection, percutaneous liver biopsy specimens showed hepatitis B virus surface antigen in the cytoplasm and hepatitis B virus core antigen in the nucleus. Four carrier animals had portal inflammatory reaction as seen in human chronic persistent hepatitis. Viral DNA was demonstrated in nucleic acid extracts of liver biopsy specimens by molecular hybridization to cloned plasmic pA01 containing hepatitis B virus DNA sequences. Although a viral molecule of length greater than the putative virus was identified, it did not appear to represent integration of viral DNA into the host genome. The chimpanzee model may serve as a means to study the mechanism of hepatitis B viral persistence and progression to chronic liver disease.