Enhanced antibody recognition with a magneto-optic surface plasmon resonance (MO-SPR) sensor
Enhanced antibody recognition with a magneto-optic surface plasmon resonance (MO-SPR) sensor
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DOI：
10.1016/j.bios.2014.02.003
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发表时间：
2014-08-15
影响因子：
12.6
通讯作者：
Rella, Roberto
中科院分区：
文献类型：
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作者：
Manera, Maria Grazia;Ferreiro-Vila, Elias;Rella, Roberto
对传统的表面等离子体共振(SPR)和磁光SPR(MOSPR)传感技术的传感性能进行了比较。MOSPR是在传统的表面等离子体共振平台的基础上发展而来的，其目的是通过施加横向配置的外加磁场来调制表面等离子体波。前人的工作表明，当这些结构中的等离子体激元共振被激发时，外加磁场会导致入射光与表面等离子体激元(SPP)的耦合发生改变。此外，当SPP被激发时，这些结构可以导致磁光(MO)活性的增强。这一现象被用来证明利用增强的分子轨道信号作为合适的换能器信号来研究生物分子在液体中相互作用的可能性。为此，用硫醇化学对传感器表面进行功能化处理，并用于记录固定在其表面的牛血清白蛋白分子与其互补靶之间的结合。与传统的SPR传感器相比，MOSPR生物传感器在灵敏度和检测下限方面具有更高的传感性能。(C)2014爱思唯尔B.V.保留所有权利。
A comparison between sensing performance of traditional SPR (Surface Plasmon Resonance) and magnetooptic SPR (MOSPR) transducing techniques is presented in this work. MOSPR comes from an evolution of traditional SPR platform aiming at modulating Surface Plasmon wave by the application of an external magnetic field in transverse configuration. Previous work demonstrated that, when the Plasmon resonance is excited in these structures, the external magnetic field induces a modification of the coupling of the incident light with the Surface Plasmon Polaritons (SPP). Besides, these structures can lead to an enhancement in the magneto-optical (MO) activity when the SPP is excited. This phenomenon is exploited in this work to demonstrate the possibility to use the enhanced MO signal as proper transducer signal for investigating biomolecular interactions in liquid phase. To this purpose, the transducer surface was functionalized by thiol chemistry and used for recording the binding between Bovine Serum Albumin molecules immobilized onto the surface and its complementary target. Higher sensing performance in terms of sensitivity and lower limit of detection of the MOSPR biosensor with respect to traditional SPR sensors is demonstrated. (c) 2014 Elsevier B.V. All rights reserved.