Immunohistochemical localization of actin: applications in surgical pathology.
Immunohistochemical localization of actin: applications in surgical pathology.
复制标题
肌动蛋白的免疫组织化学定位：在外科病理学中的应用。
DOI：
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发表时间：
1981
影响因子：
5.6
通讯作者：
J. Rosai
中科院分区：
文献类型：
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作者：
K. Mukai;J. Schollmeyer;J. Rosai
通过 Sternberger 的过氧化物酶-抗过氧化物酶方法在福尔马林固定和石蜡包埋的人体组织中证明了收缩蛋白肌动蛋白。两种类型的肌肉细胞和一些其他细胞类型均显示出阳性染色。在骨骼肌中，细胞质中弥漫着染色，交叉条纹（I 带）增强。平滑肌细胞的细胞质也被广泛染色。血管周细胞、唾液腺和乳腺肌上皮细胞，平滑肌细胞和周细胞阳性染色，界限清晰；事实证明，这种方法在研究血管病理状况（例如血管炎或肿瘤侵袭）方面优于传统的弹性组织染色。还可以评估肌上皮细胞在乳腺和唾液腺肿瘤中的参与。该方法可以与各种组织特异性标记物的其他免疫组织化学方法结合，提供有关各种软组织肿瘤的组织发生和分化的信息。
The contractile protein actin was demonstrated in formalin-fixed and paraffin-embedded human tissue by the peroxidase-antiperoxidase method of Sternberger. Both types of muscular cells and some other cell types showed positive staining. In skeletal muscle, the staining was seen diffusely in the cytoplasm with accentuation of cross-striations (I-bands). The cytoplasm of smooth muscle cells was also diffusely stained. Pericytes of blood vessels and myoepithelial cells of salivary glands and breast were clearly defined by positively stained smooth muscle cells and pericytes; this method proved superior to conventional elastic tissue staining for studying pathological conditions of blood vessels, such as vasculitides or invasion by neoplasms. Participation of myoepithelial cells in neoplasms of the breast and salivary glands could also be evaluated. This method can provide information about histogenesis and differentiation of a variety of soft-tissue neoplasms, in conjunction with other immunohistochemical methods for various tissue-specific markers.