Rice black-streaked dwarf virus P10 suppresses protein kinase C in insect vector through changing the subcellular localization of LsRACK1
Rice black-streaked dwarf virus P10 suppresses protein kinase C in insect vector through changing the subcellular localization of LsRACK1
复制标题
水稻黑条矮缩病毒P10通过改变LsRACK1的亚细胞定位抑制昆虫载体蛋白激酶C
DOI：
10.1098/rstb.2018.0315
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发表时间：
2019-03-04
影响因子：
6.3
通讯作者：
Wu, Jianxiang
中科院分区：
文献类型：
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作者：
Lu, Lina;Wang, Qi;Wu, Jianxiang
水稻黑条矮缩病毒（Rice black- stripes dwarf virus， RBSDV）是一种在自然界中由小褐飞虱（small brown planthper， SBPH）持续传播、循环传播和繁殖的病毒。在这里，我们发现RBSDV主要外衣壳蛋白（也称为P10）通过与活化蛋白激酶c1受体（LsRACK1）相互作用，抑制SBPH的蛋白激酶C （PKC）活性。P10的N端（氨基酸（aa） 1-270）和LsRACK1的C端（aa 268-315）被认为是相互作用的关键。共聚焦显微镜和亚细胞分离显示，RBSDV P10与增强的绿色荧光蛋白融合后，在frugiperda 9细胞中形成与内质网（ER）膜相关的囊泡结构。我们的研究结果还表明，RBSDV P10将LsRACK1最初的亚细胞定位从细胞质和细胞膜重定向到内质网，并影响LsRACK1激活PKC的功能。通过双链rna诱导的基因沉默抑制RACK1显著促进了RBSDV在SBPH中的复制。此外，PKC通路参与SBPH的抗病毒先天免疫应答。本研究强调RACK1通过激活PKC信号通路负向调控RBSDV在SBPH中的积累，RBSDV P10改变LsRACK1的亚细胞定位，影响其激活PKC的功能。这篇文章是主题问题“生物信号揭示智能害虫管理”的一部分。
Rice black-streaked dwarf virus (RBSDV) was known to be transmitted by the small brown planthopper (SBPH) in a persistent, circulative and propagative manner in nature. Here, we show that RBSDV major outer capsid protein (also known as P10) suppresses the protein kinase C (PKC) activity of SBPH through interacting with the receptor for activated protein kinase C 1 (LsRACK1). The N terminal of P10 (amino acids (aa) 1–270) and C terminal of LsRACK1 (aa 268–315) were mapped as crucial for the interaction. Confocal microscopy and subcellular fractionation showed that RBSDV P10 fused to enhanced green fluorescent protein formed vesicular structures associated with endoplasmic reticulum (ER) membranes in Spodoptera frugiperda nine cells. Our results also indicated that RBSDV P10 retargeted the initial subcellular localization of LsRACK1 from cytoplasm and cell membrane to ER and affected the function of LsRACKs to activate PKC. Inhibition of RACK1 by double stranded RNA-induced gene silencing significantly promoted the replication of RBSDV in SBPH. In addition, the PKC pathway participates in the antivirus innate immune response of SBPH. This study highlights that RACK1 negatively regulates the accumulation of RBSDV in SBPH through activating the PKC signalling pathway, and RBSDV P10 changes the subcellular localization of LsRACK1 and affects its function to activate PKC. This article is part of the theme issue ‘Biotic signalling sheds light on smart pest management’.