Neoflavonoids and related constituents from Nepalese propolis and their nitric oxide production inhibitory activity
Neoflavonoids and related constituents from Nepalese propolis and their nitric oxide production inhibitory activity
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DOI：
10.1021/np050009k
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发表时间：
2005-06-01
影响因子：
5.1
通讯作者：
Kadota, S
中科院分区：
文献类型：
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作者：
Awale, S;Shrestha, SP;Kadota, S
尼泊尔蜂胶的甲醇提取物产生了10个新的开链新黄酮类化合物（1-10），一个新的查尔酮（11）和8个先前报道的化合物（12-19）。根据光谱数据和化学转化确定了它们的结构。除5、8和16外，分离的化合物对脂多糖激活的巨噬细胞样J774.1细胞的一氧化氮产生具有剂量依赖性的抑制活性，并且比阳性对照n - g -单甲基-l -精氨酸更有活性（IC50, 27.1 μ M）。最有效的活性为6和7 (IC50, 0.5 μ M)，高于另一个阳性对照咖啡酸苯乙酯（IC50, 4.8 μ M）。
A methanolic extract of Nepalese propolis yielded 10 new open-chain neoflavonoids (1-10), a new chalcone (11), and eight previously reported compounds (12-19). Their structures were determined on the basis of spectroscopic data and chemical conversion. The isolated compounds other than 5, 8, and 16 showed dose-dependent inhibitory activities on nitric oxide production in lipopolysaccharide-activated macrophage-like J774.1 cells and were more active than a positive control, N-G-monomethyl-L-arginine (IC50, 27.1 mu M). The most potent activities, of 6 and 7 (IC50, 0.5 mu M), were greater than another positive control, caffeic acid phenethyl ester (IC50, 4.8 mu M).