The archaeal TFIIEα homologue facilitates transcription initiation by enhancing TATA-box recognition
The archaeal TFIIEα homologue facilitates transcription initiation by enhancing TATA-box recognition
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DOI：
10.1093/embo-reports/kve021
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发表时间：
2001-02-01
期刊：
影响因子：
7.7
通讯作者：
Jackson, SP
中科院分区：
文献类型：
--
作者：
Bell, SD;Brinkman, AB;Jackson, SP
利用重组TATA-box结合蛋白（TBP）和转录因子B (TFB)-真核TBP和tfiib的同源物-以及纯化的RNA聚合酶（RNAP），可以在体外重建许多古细菌启动子的转录。然而，迄今为止测序的所有古细菌基因组都显示存在TFE，这是真核通用转录因子TFIIE的a亚基的同源物。我们发现，虽然在体外重组系统中，TFE并不是转录所必需的，但它在某些条件下对某些启动子起刺激作用。转录反应中TATA盒的突变或TBP浓度的降低使启动子对TFE的添加敏感。相反，与TBP的饱和反应使启动子对TFE不敏感。这些结果表明，TFE促进或稳定了TBP和TATA盒之间的相互作用。
Transcription from many archaeal promoters can be reconstituted in vitro using recombinant TATA-box binding protein (TBP) and transcription factor B (TFB)-homologues of eukaryal TBP and TFIIB-together with purified RNA polymerase (RNAP). However, all archaeal genomes sequenced to date reveal the presence of TFE, a homologue of the a-subunit of the eukaryal general transcription factor, TFIIE. We show that, while TFE is not absolutely required for transcription in the reconstituted in vitro system, it nonetheless plays a stimulatory role on some promoters and under certain conditions. Mutagenesis of the TATA box or reduction of TBP concentration in transcription reactions sensitizes a promoter to TFE addition. Conversely, saturating reactions with TBP de-sensitizes promoters to TFE. These results suggest that TFE facilitates or stabilizes interactions between TBP and the TATA box.