Cellular specificity of the cure for neonatal osteopetrosis in the ia rat.
Cellular specificity of the cure for neonatal osteopetrosis in the ia rat.
复制标题
ia 大鼠新生骨石症治疗的细胞特异性。
DOI：
10.1159/000163172
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Byrnes,JE
中科院分区：
文献类型：
--
作者：
Schneider,GB;Byrnes,JE
骨质疏松症是由于破骨细胞异常导致骨吸收减少的结果。对这种突变体的研究表明，来自正常窝友的单核细胞可以治愈骨骼硬化，并在移植到体内后形成正常的破骨细胞。本研究旨在通过将21日龄正常大鼠的不同细胞群移植到不相关的新生儿/受体中，以确定这种治愈的细胞来源。通过x线摄影和测量胫骨骨髓腔的大小来评估治疗的效果。有效治疗该病的细胞悬浮液是脾、骨髓和新生儿肝脏的Ficoll-Hypaque分离物。淋巴结、胸腺和血液的Ficoll-Hypaque分离物以及腹膜细胞和脾细胞的粘附池在它们/受体中没有产生治愈。这些结果表明，治愈的细胞来源是干细胞。这一结论进一步证实了Thy 1.1抗原（大鼠的一种干细胞标记物）在大多数影响治愈的供体细胞上表达的发现。
Osteopetrosis in theia/iarat is known to be the result of reduced bone resorption due to abnormal osteoclasts. Studies in this mutant have shown that mononuclear cells from normal littermates could cure the skeletal sclerosis and result in the formation of normal osteoclasts when transplanted intoia/iarats. This investigation was pursued in an attempt to determine the cellular source of this cure by transplanting various populations of cells from 21 -day-old normal rats to unrelated newbornia/iarecipients. The effects of treatment were evaluated radiographically and by measuring the size of the tibial marrow cavity. The cellular suspensions that were effective in curing the disease were the Ficoll-Hypaque isolate of spleen, bone marrow, and newborn livers. The Ficoll-Hypaque isolates of lymph node, thymus, and blood, and the adherent pool of peritoneal cells and splenic cells did not produce a cure in theia/iarecipients. These results suggest that the cellular source of the cure is a stem cell. This conclusion is further substantiated by the finding that Thy 1.1 antigen (a stem cell marker in the rat) is expressed on a majority of the cells from the donor sources that affected a cure.