Characteristics of intestinal microbiota in the Pacific white shrimp Litopenaeus vannamei differing growth performances in the marine cultured environment
Characteristics of intestinal microbiota in the Pacific white shrimp Litopenaeus vannamei differing growth performances in the marine cultured environment
复制标题
海洋养殖环境中凡纳滨对虾不同生长性能的肠道菌群特征
DOI：
10.1016/j.aquaculture.2019.02.075
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发表时间：
2019-04-30
期刊：
影响因子：
4.5
通讯作者：
Li, Qing X.
中科院分区：
文献类型：
--
作者：
Fan, Lanfen;Li, Qing X.
太平洋白色虾凡纳滨对虾（Litopenaeus vannamei）是世界上养殖最广泛的虾，特别是在亚洲。对虾的生长性能是对虾养殖中的一个重要因素。基于16 S rRNA基因V3-V4区的Miseq测序数据，比较了对虾肠道和相关养殖场沉积物中支持不同生长性能的细菌群落。根据可分类序列鉴定出53个门。测序数据表明，在门和属的水平上，微生物群组成具有统计学显著的多样性。优势菌门为变形菌门、放线菌门、蓝藻门、拟杆菌门、绿黄杆菌门、厚壁菌门、疣微菌门和Saccharomyces细菌门。其中，放线菌和Saccharomyces细菌在对虾肠道中数量较多，且生长性能正常，这些门可能与对虾的免疫和消化功能有关。变形菌在生长缓慢的虾肠中数量较多，增加的变形菌数量是潜在的致病风险。在属水平上，norank _f_Propionibacteriaceae、Ruegeria、Robiginitalea、unclassified_c_Actinobacteria和norank_f_TM146在0.001
The Pacific white shrimp Litopenaeus vannamei is the most widely cultivated shrimp in the world, particularly in Asia. The growth performance is a vital factor in shrimp farming. In the present study, comparisons were made among the bacterial communities in shrimp intestines and the related marine farm sediments that supported different growth performances, based on Miseq sequencing data of the V3-V4 region of 16S rRNA gene. Fifty three phyla were identified with the classifiable sequence. Sequencing data demonstrated a statistically significant diversity in microbiota compositions at the phylum and genus levels. The dominant phyla were Proteobacteria, Actinobacteria, Cyanobacteria, Bacteroidetes, Chloroflexi, Firmicutes, Verrucomicrobia, and Saccharibacteria. Of which, Actinobacteria and Saccharibacteria were more abundant in the shrimp intestines with a normal growth performance, these phyla may be related with shrimp immunity and digestion. Proteobacteria were more abundant in shrimp intestines with slow growth performance, an increased abundance of Proteobacteria was a potential risk of disease. At the genus level, norank _f_Propionibacteriaceae, Ruegeria, Robiginitalea, unclassified_c_Actinobacteria and norank_f_TM146 exhibited extremely statistically significant differences at 0.001 < P