Severe chronic graft-versus-host disease is characterized by a preponderance of CD4(+) effector memory cells relative to central memory cells.
Severe chronic graft-versus-host disease is characterized by a preponderance of CD4(+) effector memory cells relative to central memory cells.
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DOI：
10.1182/blood-2003-09-3286
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发表时间：
2004-05
期刊：
影响因子：
20.3
通讯作者：
K. Yamashita;U. Choi;Patricia C. Woltz;Susan F. Foster;M. Sneller;F. Hakim;D. Fowler;M. Bishop;S. Pavletic;M. Tamari;K. Castro;A. Barrett;R. Childs;G. Illei;S. Leitman;H. Malech;M. Horwitz
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文献类型：
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作者：
K. Yamashita;U. Choi;Patricia C. Woltz;Susan F. Foster;M. Sneller;F. Hakim;D. Fowler;M. Bishop;S. Pavletic;M. Tamari;K. Castro;A. Barrett;R. Childs;G. Illei;S. Leitman;H. Malech;M. Horwitz
供体同种异体CD4(+) T细胞在慢性移植物抗宿主病（cGVHD）的发病机制中起重要作用，但导致GVHD的CD4(+) T细胞的特定亚群尚未确定。我们假设cGVHD可能与CD4（+）效应记忆细胞(CCR7（-)/CD62L（低），CD4(EM)）的优势有关。我们分析了发生或未发生cGVHD的干细胞移植患者和健康供者CD4(+) T细胞上CCR7和CD62L的表达。cGVHD患者CD4（EM）细胞百分比（35.5% +/- 2.9%）高于健康供者（13.8% +/- 0.7%,P < 0.0001）或接受移植的无cGVHD患者（21.7% +/- 2.1%,P < 0.01）。使用皮质类固醇剂量作为cGVHD严重程度的替代标记，严重的cGVHD与较高的CD4（EM）细胞百分比相关。在皮质类固醇依赖的系统性狼疮红斑或韦格纳肉芽肿患者中，CD4（EM）细胞的比例与接受移植的无cGVHD患者没有差异。这一发现表明CD4（EM）细胞的过度表达是cGVHD的一个独特特征。
Donor alloreactive CD4(+) T cells are important to the pathogenesis of chronic graft-versus-host disease (cGVHD), but specific subsets of CD4(+) T cells responsible for GVHD have not been defined. We hypothesized that cGVHD might be associated with a preponderance of CD4(+) effector memory cells (CCR7(-)/CD62L(low), CD4(EM)). We analyzed CCR7 and CD62L expression on CD4(+) T cells from stem cell transplantation patients, who did or did not develop cGVHD, and healthy donors. Patients with cGVHD had a higher percentage of CD4(EM) cells (35.5% +/- 2.9%) than healthy donors (13.8% +/- 0.7%; P <.0001) or patients without cGVHD that received a transplant (21.7% +/- 2.1%; P <.01). Using corticosteroid dose as a surrogate marker for cGVHD severity, severe cGVHD was associated with a higher percentage of CD4(EM) cells. The proportion of CD4(EM) cells in corticosteroid-dependent patients with systemic lupus erythematosis or Wegener granulomatosis did not differ from patients without cGVHD that received a transplant. This finding implies that overrepresentation of CD4(EM) cells is a unique feature of cGVHD.