High-mobility group A1 proteins are overexpressed in human leukaemias
High-mobility group A1 proteins are overexpressed in human leukaemias
复制标题
DOI：
10.1042/bj20021493
复制
发表时间：
2003-05-15
影响因子：
4.1
通讯作者：
Fusco, A
中科院分区：
文献类型：
--
作者：
Pierantoni, GM;Agosti, V;Fusco, A
高迁移率 A 组 (HMGA) 蛋白是结合 DNA 和多种转录因子的非组蛋白核蛋白。它们参与染色质结构和功能的调节。 HMGA 蛋白在正常成人组织中表达较低，但在胚胎发育和多种人类肿瘤中表达丰富。 HMGA 基因的重排经常在间质来源的人类良性肿瘤中检测到，例如肿瘤。 lipomas, lung hamartomas and uterine leiomiomas. HMGA 蛋白参与控制前脂肪细胞系 3T3-L1 的细胞生长和分化。为了更好地了解 HMGA1 蛋白在血液肿瘤和造血细胞分化中的作用，我们研究了它们在人类白血病和诱导终末分化的白血病细胞系中的表达。在这里，我们报告了 HMGA I 在不同来源的大多数新鲜人类白血病和几种白血病细胞系中的过度表达。此外，三种细胞系向巨核细胞表型的分化与 HMGA1 蛋白诱导相关，而红细胞和单核细胞分化的诱导通常导致 HMGA1 表达减少。
High-mobility group A (HMGA) proteins are non-histone nuclear proteins that bind DNA and several transcription factors. They are involved in the regulation of chromatin structure and function. HMGA protein expression is low in normal adult tissues, but abundant during embryonic development and in several human tumours. Rearrangements of the HMGA genes have been frequently detected in human benign tumours of mesenchymal origin, e.g. lipomas, lung hamartomas and uterine leiomiomas. HMGA proteins have been implicated in the control of cell growth and differentiation of the pre-adipocytic cell line 3T3-L1. In an attempt to better understand the role of HMGA1 proteins in haematological neoplasias and in the differentiation of haematopietic cells, we have investigated their expression in human leukaemias and in leukaemic cell lines induced to terminal differentiation. Here we report HMGA I overexpression in most fresh human leukaemias of different origin and in several leukaemic cell lines. Moreover, differentiation of three cell lines towards the megakaryocytic phenotype was associated with HMGA1 protein induction, whereas induction of erythroid and monocytic differentiation generally resulted in reduced HMGA1 expression.