Anginex-conjugated liposomes for targeting of angiogenic endothelial cells
Anginex-conjugated liposomes for targeting of angiogenic endothelial cells
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DOI：
10.1021/bc060316h
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发表时间：
2007-05-01
影响因子：
4.7
通讯作者：
Griffioen, Arjan W.
中科院分区：
文献类型：
--
作者：
Brandwijk, Ricardo J. M. G. E.;Mulder, Willem J. M.;Griffioen, Arjan W.
识别肿瘤血管生成特异性配体将允许靶向肿瘤血管系统。脂质载体可用于输送治疗疾病的治疗剂或用于分子成像的造影剂。靶向配基将允许将这种制剂特定地输送到血管生成部位，从而减少副作用并提高效率。Anginex是一种人工合成的33聚体血管抑制肽，已被描述为具有血管生成活性的内皮细胞，是理想的靶向配体。为了研究Anginex的这一应用，将荧光标记的顺磁性脂质体与Anginex偶联。利用相差显微镜和荧光显微镜以及磁共振成像(MRI)，我们证明了血管紧张素转运蛋白偶联脂质体与激活的内皮细胞特异性结合，建议将其作为血管生成靶向试剂应用于血管生成依赖性疾病的分子靶向和分子成像。
Identification of a tumor angiogenesis specific ligand would allow targeting of tumor vasculature. Lipidic vehicles can be used to deliver therapeutic agents for treatment of disease or contrast agents for molecular imaging. A targeting ligand would allow specific delivery of such formulations to angiogenic sites, thereby reducing side effects and gaining efficiency. Anginex, a synthetic 33-mer angiostatic peptide, has been described to home angiogenically activated endothelium, suggesting an ideal candidate as targeting ligand. To investigate this application of anginex, fluorescently labeled paramagnetic liposomes were conjugated with anginex. Using phase contrast and fluorescence microscopy as well as magnetic resonance imaging (MRI), we demonstrate that anginex-conjugated liposomes bind specifically to activated endothelial cells, suggesting application as an angiogenesis targeting agent for molecular targeting and molecular imaging of angiogenesis-dependent disease.