Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo
Downregulation of TRIM27 expression inhibits the proliferation of ovarian cancer cells in vitro and in vivo
复制标题
TRIM27表达下调抑制卵巢癌细胞体内外增殖
DOI：
10.1038/labinvest.2015.132
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发表时间：
2016-01-01
影响因子：
5
通讯作者：
Wang, Xiaoyan
中科院分区：
文献类型：
--
作者：
Ma, Yanyan;Wei, Zengtao;Wang, Xiaoyan
TRIM27（包含三部分基序的27）最初被鉴定为与RET（转染期间重新排列）原癌基因的融合伴侣，并在多种肿瘤细胞和组织中高表达。然而，TRIM27在卵巢癌中的表达水平和功能仍不清楚。在这里，我们测量了正常卵巢和输卵管上皮细胞以及卵巢浆液性癌细胞中TRIM27的表达，并将TRIM27表达与临床和病理参数相关联。此外，我们还检测了 TRIM27 敲低对细胞培养物和异种移植物中卵巢癌细胞增殖的影响。结果表明，TRIM27在卵巢浆液性癌细胞中高表达，且TRIM27表达与卵巢浆液性癌患者的转移及FIGO分期显着相关。 TRIM27表达下调可抑制细胞培养物中卵巢癌细胞的增殖，并抑制裸鼠异种移植物的生长。 TRIM27 敲除通过上调 p-P38 的表达并下调 p-AKT 的表达来诱导卵巢癌细胞的细胞周期停滞和凋亡。因此，本研究表明TRIM27可能作为癌基因在卵巢癌的发展过程中发挥重要作用，并可作为诊断和治疗靶点。
TRIM27 (tripartite motif-containing 27) was originally identified as a fusion partner with the RET (REarranged during transfection) proto-oncogene and is highly expressed in various tumor cells and tissues. However, the level of expression and function of TRIM27 in ovarian cancer remain unclear. Here we have measured the expression of TRIM27 in normal ovarian and fallopian tube epithelial cells and in ovarian serous carcinoma cells and correlated TRIM27 expression with clinical and pathological parameters. In addition, we detected the effect of TRIM27 knockdown on proliferation of ovarian cancer cells in cell culture and xenografts. The results demonstrated that TRIM27 was highly expressed in ovarian serous carcinoma cells, and TRIM27 expression was significantly correlated with metastasis and FIGO stage in ovarian serous carcinoma patients. Downregulation of TRIM27 expression suppressed the proliferation of ovarian cancer cells in cell culture and inhibited the growth of xenografts in nude mice. TRIM27 knockdown induced cell cycle arrest and apoptosis in ovarian cancer cells by upregulating the expression of p-P38 and downregulating the expression of p-AKT. Thus the present study suggests that TRIM27 could have important roles as an oncogene during the development of ovarian cancer and could serve as a diagnostic and therapeutic target.