Dual lifetime referenced optical sensor membrane for the determination of copper(II) ions
Dual lifetime referenced optical sensor membrane for the determination of copper(II) ions
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DOI：
10.1016/s0003-2670(02)00234-9
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发表时间：
2002-06-26
影响因子：
6.2
通讯作者：
Werner, T
中科院分区：
文献类型：
--
作者：
Mayr, T;Klimant, I;Werner, T
传感器膜已被开发用于测定铜（II）离子，由于发光强度的内部参考显示出优异的性能。所应用的传感方案（双寿命参考）利用指示剂荧光黄（LY）和惰性参考发光体（包埋在聚丙烯腈珠中的钌络合物）。两者都包含在水凝胶基质中。LY的铜依赖性荧光强度变化可以转换为相移或时间依赖性参数。该传感膜能够在中性或弱酸性条件下在1至1000 μ M的动态范围内以出色的高选择性测定铜（II）。通过对混浊溶液的测量，证明了参比方法优于基于强度的测量。该方案也适用于二维测量的时域。传感器集成的微量滴定板用CCD照相机成像，该CCD照相机用微秒范围内的方脉冲选通。(C)2002 Elsevier Science B.V.保留所有权利。
A sensor membrane has been developed for the determination of copper(II) ions that displays excellent performance due to internal referencing of luminescence intensities. The applied sensing scheme (dual lifetime referencing) makes use of the indicator lucifer yellow (LY) and an inert reference luminophore (a ruthenium complex entrapped in polyacrylonitrile beads). Both are contained in a hydrogel matrix. The copper-dependent fluorescence intensity change of LY can be converted in either a phase shift or time dependent parameter. The sensing membrane is capable of determining copper(II) with an outstanding high selectivity over a dynamic range between 1 and 1000 muM in neutral or weekly acidic conditions. The advantages of the referencing method over intensity based measurements was demonstrated by the measurement of turbid solutions. The scheme was also applied to two-dimensional measurements in the time domain. Sensor integrated microtiterplates were imaged with a CCD camera gated with square pulses in the microsecond range. (C) 2002 Elsevier Science B.V. All rights reserved.