GROWTH OF MOUSE BONE MARROW CELLS IN VITRO
GROWTH OF MOUSE BONE MARROW CELLS IN VITRO
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DOI：
10.1038/icb.1966.28
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发表时间：
1966-01-01
期刊：
影响因子：
--
通讯作者：
METCALF, D
中科院分区：
文献类型：
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作者：
BRADLEY, TR;METCALF, D
一个简单的体外技术描述了从小鼠骨髓单细胞悬浮液的集落生长。该系统包括将骨髓细胞在琼脂中接种在其他细胞的饲养层上，来自8天大的小鼠肾脏和17天的小鼠胚胎的那些细胞被证明是最有效的饲养层类型。使用肾细胞饲养层，每1X1016个有核骨髓细胞生长约400个集落。证明了接种的细胞数与形成的集落数之间的线性关系。与骨髓细胞相比，淋巴结或胸腺细胞不形成集落，但使用脾细胞形成少量集落。在集落发育的早期，优势细胞类型是一个大的单核细胞，细胞质中充满颗粒，甲苯胺蓝异染染色。随着殖民地的生长，出现了具有环形或马蹄形核的细胞，并且随着菌落的进一步生长，观察到具有类似于多形态血细胞的分段核的较小细胞的进展。
A simple in-vitro technique is described for the growth of colonies from single cell suspensions of mouse bone marrow. The system involves the plating of marrow cells in agar on feeder layers of other cells, those from 8-day-old mouse kidney and 17th day mouse embryo being shown to be the most efficient type of feeder layers. Approximately 400 colonies per 1X1016 nucleated marrow cells were grown, using kidney cell feeder layers. A linear relationship between the number of cells plated and the number of colonies developing was demonstrated. In comparison with the marrow cells, lymph node or thymus cells did not form colonies, but a small number of colonies was formed using spleen cells. Early in the development of the colonies the dominant cell type was a large mononuclear cell with cytoplasm filled with granules staining metachromatically with toluidine blue. With growth of the colony, cells with ring or horseshoe-shaped nuclei appeared, and a progression with further colony growth to smaller cells with segmented nuclei similar to poly-morphonuclear blood cells was observed.