Two functional domains conserved in major and alternate bacterial sigma factors
Two functional domains conserved in major and alternate bacterial sigma factors
复制标题
主要和替代细菌西格玛因子中保守的两个功能域
DOI：
10.1016/0014-5793(85)81203-5
复制
发表时间：
1985
期刊：
影响因子：
3.5
通讯作者：
J. Bouvier
中科院分区：
文献类型：
--
作者：
P. Stragier;C. Parsot;J. Bouvier
将大肠杆菌和枯草芽孢杆菌的σ因子序列与两种σ样蛋白的序列进行比对，这两种σ样蛋白是参与大肠杆菌中热激基因表达的HtpR和B.枯草杆菌。内部区域在四种蛋白质中高度保守，并且被提出参与σ因子与核心RNA聚合酶的结合。这四种蛋白质的羧基末端部分呈现出在几种原核DNA结合蛋白中发现的特征结构，并被认为参与启动子识别。
Sequences of the sigma factors ofEscherichia coliandBacillus subtiliswere aligned with the sequences of two sigma-like proteins, HtpR, involved in the expression of heat-shock genes inE.coli, and SpoIIG, necessary for endospore formation inB. subtilis. An internal region is highly conserved in the four proteins and is proposed to be involved in binding of sigma factors to core RNA polymerase. The carboxy-terminal part of the four proteins presents the characteristic structure found in several prokaryotic DNA-binding proteins and is proposed to be involved in promoter recognition.